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RESEARCH PRODUCT
Subcellular duplex DNA and G‐quadruplex interaction profiling of a hexagonal PtII metallacycle
Bernhard EnglingerBernhard K. KepplerAlessio TerenziAlessio TerenziChristine PirkerJohannes M. ReiseckerCarlos PeinadorClaudia KielerGerald TimelthalerOlaya DomarcoCarina DinhofMarcos GarciaWalter Bergersubject
KeratinocytesModels MolecularOrganoplatinum CompoundsmetallacycleSupramolecular chemistry010402 general chemistryG-quadruplex01 natural sciencesCatalysisEpitopeMetallacycleCell Line Tumorsubcellular localizationHumansplatinumPlatinumG-quadruplex010405 organic chemistryHexagonal crystal systemChemistrySubcellular localizationCommunicationDNAGeneral ChemistryFibroblastsMetallacycleSubcellular localizationPhotobleachingCommunicationsSCC0104 chemical sciencesChromatinG-QuadruplexesSettore CHIM/03 - Chimica Generale E InorganicaMCF-7 CellsBiophysicsSpectrophotometry Ultravioletdescription
[Abstract] Metal‐driven self‐assembly afforded a multitude of fascinating supramolecular coordination complexes (SCCs) with applications as catalysts, host–guest, and stimuli‐responsive systems. However, the interest in the biological applications of SCCs is only starting to emerge and thorough characterization of their behavior in biological milieus is still lacking. Herein, we report on the synthesis and detailed in‐cell tracking of a Pt2L2 metallacycle. We show that our hexagonal supramolecule accumulates in cancer cell nuclei, exerting a distinctive blue fluorescence staining of chromatin resistant to UV photobleaching selectively in nucleolar G4‐rich regions. SCC co‐localizes with epitopes of the quadruplex‐specific antibody BG4 and replaces other well‐known G4 stabilizers. Moreover, the photophysical changes accompanying the metallacycle binding to G4s in solution (fluorescence quenching, absorption enhancement) also take place intracellularly, allowing its subcellular interaction tracking. Ministerio de Economía, Industria y Competitividad; CTQ2016-75629-P
year | journal | country | edition | language |
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2019-01-01 |