6533b7defe1ef96bd12766bc
RESEARCH PRODUCT
The Norway spruce genome sequence and conifer genome evolution
Stefan JanssonYao-cheng LinBo ZhangMelis KucukogluAndrea ZuccoloBjörn SundbergLydia GramzowKermit RitlandJean BousquetPär K. IngvarssonYves Van De PeerHannele TuominenMalin ElfstrandAndrey AlexeyenkoStefania GiacomelloRosario Garcia GilTotte NiittyläJohn MackayKevin VannesteCarlos Talavera-lópezNathaniel R. StreetBjörn AnderssonPieter J. De JongLars ArvestadLars ArvestadLisa KlassonNicolas DelhommeRishikesh P. BhaleraoGünter TheißenFredrik LysholmJimmie HällmanAnna WetterbomJosep A. RossellóMaxim KoriabineJuliana Stival SenaJohannes LuthmanEllen SherwoodRiccardo VicedominiDouglas G. ScofieldZhiqiang WuNemanja RilakovicMichele MorganteStacey Lee ThompsonJoerg BohlmannTorgeir R. HvidstenTorgeir R. HvidstenKristina HolmbergThomas SvenssonKristoffer SahlinPhilipp ZerbeMax KällerBjörn NystedtOlivier KeechCarol RitlandFrancesco VezziJoakim LundebergÅKe OlsonOve Nilssonsubject
Transposable elementGenome evolutionRNA UntranslatedTranscription GeneticRECOMBINATIONGenomicsGENE FAMILYGenes PlantSEED PLANTSGenomeLONG NONCODING RNASSIZE VARIATIONEvolution MolecularGymnospermBotanyNaturvetenskapGene SilencingRICEPiceaGenome sizePINUSConserved SequenceWhole genome sequencingInternetMultidisciplinarybiologyTerminal Repeat SequencesBiology and Life SciencesPicea abiesGenomicsSequence Analysis DNALINEAGEbiology.organism_classificationIntronsPhenotypeDNA Transposable ElementsTRANSPOSABLE ELEMENTSORYZA-SATIVANatural SciencesGenome Plantdescription
Conifers have dominated forests for more than 200 million years and are of huge ecological and economic importance. Here we present the draft assembly of the 20-gigabase genome of Norway spruce (Picea abies), the first available for any gymnosperm. The number of well-supported genes (28,354) is similar to the >100 times smaller genome of Arabidopsis thaliana, and there is no evidence of a recent whole-genome duplication in the gymnosperm lineage. Instead, the large genome size seems to result from the slow and steady accumulation of a diverse set of long-terminal repeat transposable elements, possibly owing to the lack of an efficient elimination mechanism. Comparative sequencing of Pinus sylvestris, Abies sibirica, Juniperus communis, Taxus baccata and Gnetum gnemon reveals that the transposable element diversity is shared among extant conifers. Expression of 24-nucleotide small RNAs, previously implicated in transposable element silencing, is tissue-specific and much lower than in other plants. We further identify numerous long (>10,000 base pairs) introns, gene-like fragments, uncharacterized long non-coding RNAs and short RNAs. This opens up new genomic avenues for conifer forestry and breeding.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2013-05-01 |