6533b81ffe1ef96bd1277b28

RESEARCH PRODUCT

Rapid characterization of four species of the Saccharomyces sensu stricto complex according to mitochondrial DNA patterns

Amparo QuerolJosé Manuel GuillamónT. HuertaEladio Barrio

subject

GeneticsMitochondrial DNAImmunologySaccharomyces cerevisiaeSaccharomyces bayanusBiologySaccharomyces pastorianusbiology.organism_classificationMicrobiologySaccharomycesParadoxusDNA MitochondrialRestriction fragmentSaccharomycesbiology.proteinSaccharomyces paradoxusDNA Fungal

description

Several strains of the four sibling species of the genus Saccharomyces (S. bayanus, S. cerevisiae, S. paradoxus, and S. pastorianus) were characterized by using a rapid and simple method of restriction analysis of mitochondrial DNA. Patterns obtained with four-cutter endonucleases (such as AluI, DdeI, HinfI, and RsaI) made it possible to differentiate each species. S. cerevisiae and S. paradoxus presented a greater number of large fragments than S. pastorianus and S. bayanus with all the assay enzymes. With AluI and DdeI, species-specific bands clearly permitted differentiation between S. pastorianus and S. bayanus. To test the resolution of this method, wild Saccharomyces strains were analyzed. The correct assignment of these strains to a known taxon by this rapid method was confirmed by means of electrophoretic karyotyping.

yearjournalcountryeditionlanguage
1994-10-01
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