6533b81ffe1ef96bd12787b3

RESEARCH PRODUCT

Comparative relaxant effects of the NO donors sodium nitroprusside, DEA/NO and SPER/NO in rabbit carotid arteries.

Enrique AlborchGermán TorregrosaJuan B. SalomJosé M. CentenoMarta OrtíMaría D. Barberá

subject

MaleNitroprussidemedicine.medical_specialtyVasodilator AgentsNitric oxidePotassium Chloridechemistry.chemical_compoundInternal medicinemedicineAnimalsNitric Oxide DonorsCyclic GMPPharmacologyLagomorphabiologyDose-Response Relationship Drugbiology.organism_classificationmedicine.anatomical_structureEndocrinologyCarotid ArteriesHydrazineschemistryAnesthesiaCirculatory systemNitrogen OxidesSpermineSodium nitroprussideRabbitsMethylene blueHistamineBlood vesselArterymedicine.drug

description

1. Sodium nitroprusside (SNP, 10(-9)-3x10(-4) M), diethylamine/NO complex (DEA/NO, 10(-9)-10(-4) M) and spermine/NO complex (SPER/NO, 10(-8)-3x10(-4) M) induced concentration-dependent relaxation of isolated rabbit carotid arteries precontracted with KCl (50 mM) or with histamine (3x10(-6) M). 2. In KCl-precontracted arteries the order of potency was SNP=DEA/NO>SPER/NO, and in histamine-precontracted arteries the order of potency was SNP>DEA/NO>SPER/NO. Relaxations to the three NO donors were significantly higher in histamine-precontracted arteries than in KCl-precontracted arteries. 3. The guanylyl cyclase inhibitor methylene blue (10(-5) M) significantly inhibited relaxations to the three NO donors in histamine-precontracted arteries and, to a lesser extent, in KCl-precontracted arteries. 4. In conclusion, the NO donors SNP, DEA/NO and SPER/NO induce quantitatively different relaxation of rabbit carotid artery. Both, lower relaxant effects in depolarized arteries and inhibition of relaxation by methylene blue indicate the mediation of cGMP formation in the relaxant effects of the three NO donors.

yearjournalcountryeditionlanguage
1999-01-15General pharmacology
10.1016/s0306-3623(98)00087-1https://pubmed.ncbi.nlm.nih.gov/9888258