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RESEARCH PRODUCT

Centenarians Overexpress Pluripotency-Related Genes.

Cristina Mas BarguesJose ViñaAnder MatheuConsuelo BorrasPaula SanchisAlejandro Berna-erroMarta InglesJuan-antonio Avellana

subject

0301 basic medicineAdultMalePluripotent Stem CellsAgingCellPeripheral blood mononuclear cellCohort Studies03 medical and health sciencesKruppel-Like Factor 40302 clinical medicineSOX2MedicineHumansGeneCells CulturedAged 80 and overbusiness.industryMesenchymal stem cellAge FactorsMembrane ProteinsReverse transcription polymerase chain reaction030104 developmental biologymedicine.anatomical_structureGene Expression RegulationKLF4Cancer researchLeukocytes MononuclearFemaleGeriatrics and GerontologyCentenarianbusiness030217 neurology & neurosurgery

description

Abstract Human mesenchymal cells can become pluripotent by the addition of Yamanaka factors OCT3/4, SOX2, c-MYC, KLF4. We have recently reported that centenarians overexpress BCL-xL, which has been shown to improve pluripotency; thus, we aimed to determine the expression of pluripotency-related genes in centenarians. We recruited 22 young, 32 octogenarian, and 47 centenarian individuals and determined the mRNA expression of Yamanaka factors and other stemness-related cell surface marker genes (VIM, BMP4, NCAM, BMPR2) in peripheral blood mononuclear cells by reverse transcription polymerase chain reaction. We found that centenarians overexpress OCT3/4, SOX2, c-MYC, VIM, BMP4, NCAM, and BMPR2, when compared with octogenarians (p < .05). We further tested the functional role of BCL-xL in centenarians’ ability to express pluripotency-related genes: lymphocytes from octogenarians transduced with BCL-xL overexpressed SOX2, c-MYC, and KLF4. We conclude that centenarians overexpress Yamanaka Factors and other stemness-related cell surface marker genes, which may contribute to their successful aging.

yearjournalcountryeditionlanguage
2018-04-30The journals of gerontology. Series A, Biological sciences and medical sciences
10.1093/gerona/gly168https://pubmed.ncbi.nlm.nih.gov/30052797