6533b820fe1ef96bd12792a7
RESEARCH PRODUCT
Feeding enhances extracellular lactate of local origin in the rostromedial hypothalamus but not in the cerebellum.
Arnauld-yves GouchamStylianos Nicolaidissubject
Blood GlucoseMaleMicrodialysismedicine.medical_specialtyCerebellumTime FactorsMicrodialysisCentral nervous systemBiologyDeoxyglucoseBlood–brain barrierEatingInternal medicineCerebellumExtracellular fluidmedicineExtracellularAnimalsLactic AcidRats WistarMolecular BiologyGeneral Neurosciencedigestive oral and skin physiologyMetabolismPostprandial PeriodRatsmedicine.anatomical_structureEndocrinologyGlucosenervous systemHypothalamusVentromedial Hypothalamic NucleusNeurology (clinical)Extracellular SpaceDevelopmental BiologyParaventricular Hypothalamic Nucleusdescription
Abstract The use of brain microdialysis together with chronic vascular catheterization allowed us to assay extracellular fluid lactate (ECF L ) in both the ventromedial–paraventricular (VMH–PVN) area of the hypothalamus and the cerebellum, in parallel with measures of plasma levels, and in relation to food intake. A 45 min scheduled meal increased VMH–PVN ECF L by 28%. This increase was not observed in the cerebellum. The prandial increase in plasma glucose (43%, from 4.74 to 6.77 mM) and lactate (84%, from 0.83 to 1.53 mM) showed a different temporal pattern and lasted longer than that of the ECF L . Glucose delivery by reverse dialysis for 45 min into the VMH–PVN area increased ECF L by 49%. When local glucose utilization was prevented by reverse dialysis-delivered 2-deoxy- d -glucose (2-DG), not only did VMH–PVN ECF L drop, but the feed-related increase in ECF L was blocked without affecting the normal rise in plasma glucose and in lactate. These results indicate that meal-related ECF L production and variations are independent of circulating lactate, but may depend on substrate availability in these hypothalamic structures.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1999-01-08 | Brain research |