6533b820fe1ef96bd1279a70

RESEARCH PRODUCT

Recombinant virus-like particles of a norovirus (genogroup II strain) administered intranasally and orally with mucosal adjuvants LT and LT(R192G) in BALB/c mice induce specific humoral and cellular Th1/Th2-like immune responses

Béatrice Nicollier-jamotLionel PirothPierre PothierAgathe OgierEvelyne Kohli

subject

Cellular immunityvirusesmedicine.medical_treatmentAdministration OralEnzyme-Linked Immunosorbent AssayAntibodies ViralBALB/cMicrobiologyFecesMiceTh2 CellsImmune systemAdjuvants ImmunologicVirus-like particlemedicineAnimalsAdministration IntranasalCells CulturedImmunity CellularMice Inbred BALB CVaccines SyntheticGeneral VeterinaryGeneral Immunology and MicrobiologybiologyNorovirusPublic Health Environmental and Occupational HealthViral VaccinesTh1 Cellsbiology.organism_classificationVirologyInfectious DiseasesCytokineAntibody FormationHumoral immunitybiology.proteinMolecular MedicineFemaleLymph NodesAntibodyAdjuvantSpleen

description

We investigated the immune response induced by mucosal immunization of BALB/c mice with virus-like particles (VLPs) of a genogroup II norovirus, Dijon171/96 virus, produced in the baculovirus system. VLPs administered alone by the intranasal route induced a high serum antibody response as well as fecal IgA, which were enhanced when the heat-labile Escherichia coli toxin or its non toxic mutant LT(R192G) was coadministered. In these conditions, the oral route was also efficient. Cytokine production by cells from different lymphoid tissues was then assessed after in vitro restimulation. A Th1/Th2-like response was observed in cervical lymph node and Peyer's patch (PP) cell cultures from mice intranasally or orally immunized with either adjuvant indicating that, on the assumption that T cells are the primary cells producing the cytokines after in vitro restimulation, specific T lymphocytes are present in the intestine after intranasal immunization.

yearjournalcountryeditionlanguage
2003-01-23Vaccine
https://doi.org/10.1016/j.vaccine.2003.10.004