0000000000465728

AUTHOR

Béatrice Nicollier-jamot

showing 2 related works from this author

Recombinant virus-like particles of a norovirus (genogroup II strain) administered intranasally and orally with mucosal adjuvants LT and LT(R192G) in…

2003

We investigated the immune response induced by mucosal immunization of BALB/c mice with virus-like particles (VLPs) of a genogroup II norovirus, Dijon171/96 virus, produced in the baculovirus system. VLPs administered alone by the intranasal route induced a high serum antibody response as well as fecal IgA, which were enhanced when the heat-labile Escherichia coli toxin or its non toxic mutant LT(R192G) was coadministered. In these conditions, the oral route was also efficient. Cytokine production by cells from different lymphoid tissues was then assessed after in vitro restimulation. A Th1/Th2-like response was observed in cervical lymph node and Peyer's patch (PP) cell cultures from mice …

Cellular immunityvirusesmedicine.medical_treatmentAdministration OralEnzyme-Linked Immunosorbent AssayAntibodies ViralBALB/cMicrobiologyFecesMiceTh2 CellsImmune systemAdjuvants ImmunologicVirus-like particlemedicineAnimalsAdministration IntranasalCells CulturedImmunity CellularMice Inbred BALB CVaccines SyntheticGeneral VeterinaryGeneral Immunology and MicrobiologybiologyNorovirusPublic Health Environmental and Occupational HealthViral VaccinesTh1 Cellsbiology.organism_classificationVirologyInfectious DiseasesCytokineAntibody FormationHumoral immunitybiology.proteinMolecular MedicineFemaleLymph NodesAntibodyAdjuvantSpleenVaccine
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Molecular Cloning, Expression, Self-Assembly, Antigenicity, and Seroepidemiology of a Genogroup II Norovirus Isolated in France

2003

ABSTRACT Virus-like particles of Dijon171/96 virus, a genogroup II norovirus, were expressed in a baculovirus system and were used for a seroepidemiological study of 1,078 age-stratified human sera collected in Dijon, France. The results showed a seroprevalence of 74.1%. Furthermore, we showed that murine antibodies generated against recombinant Dijon171/96 virus, and human antibodies recognized discontinuous epitopes on the particles.

AdultMicrobiology (medical)AntigenicityAdolescentvirusesMolecular Sequence DataEnzyme-Linked Immunosorbent AssayAntibodies Viralmedicine.disease_causeEpitopeVirusSerologyMiceVirus-like particleSeroepidemiologic StudiesVirologymedicineAnimalsHumansSeroprevalenceCloning MolecularSerotypingChildAgedCaliciviridae InfectionsbiologyInfantMiddle Agedbiology.organism_classificationVirologyCaliciviridaeChild PreschoolImmunoglobulin GSpectrometry Mass Matrix-Assisted Laser Desorption-IonizationNorovirusCapsid ProteinsElectrophoresis Polyacrylamide GelFranceCaliciviridaeJournal of Clinical Microbiology
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