Molecular Cloning, Expression, Self-Assembly, Antigenicity, and Seroepidemiology of a Genogroup II Norovirus Isolated in France

6533b862fe1ef96bd12c62bd

RESEARCH PRODUCT

Molecular Cloning, Expression, Self-Assembly, Antigenicity, and Seroepidemiology of a Genogroup II Norovirus Isolated in France

Valérie Pico Pierre Pothier Béatrice Nicollier-jamot Evelyne Kohli

subject

Adult Microbiology (medical)Antigenicity Adolescent viruses Molecular Sequence Data Enzyme-Linked Immunosorbent Assay Antibodies Viral medicine.disease_cause Epitope Virus Serology Mice Virus-like particle Seroepidemiologic Studies Virology medicine Animals Humans Seroprevalence Cloning Molecular Serotyping Child Aged Caliciviridae Infections biology Infant Middle Aged biology.organism_classification Virology Caliciviridae Child Preschool Immunoglobulin G Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Norovirus Capsid Proteins Electrophoresis Polyacrylamide Gel France Caliciviridae

description

ABSTRACT Virus-like particles of Dijon171/96 virus, a genogroup II norovirus, were expressed in a baculovirus system and were used for a seroepidemiological study of 1,078 age-stratified human sera collected in Dijon, France. The results showed a seroprevalence of 74.1%. Furthermore, we showed that murine antibodies generated against recombinant Dijon171/96 virus, and human antibodies recognized discontinuous epitopes on the particles.

year	journal	country	edition	language
2003-08-01	Journal of Clinical Microbiology

https://doi.org/10.1128/jcm.41.8.3901-3904.2003

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