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RESEARCH PRODUCT
MiRNA profiling by NGS in resectable non-small cell lung cancer: Prognostic implications.
Sandra GallachCarlos CampsDavid MontanerRicardo GuijarroEloisa Jantus-lewintreSilvia CalabuigE. SanmartinAna BlascoRafael SireraMarta UsóMiguel Martorellsubject
Cancer ResearchIn silicoRNAComputational biologyBiologyBioinformaticsmedicine.diseaseMiRBaseOncologymicroRNAGene expressionmedicineMirna profilingNon small cellLung cancerdescription
7559 Background: MicroRNAs (miRNA) regulate gene expression, and are implicated in several processes like tumorogenesis. Here, we applied a multiplexed NGS approach to study differentially expressed miRNAs (tumor/ normal) in a cohort of resectable NSCLC patients and its correlation with clinical outcome. Methods: RNA was isolated from frozen lung specimens (tumor/ normal) from 33 patients. High-quality samples were analyzed and enriched in the miRNA fraction. Libraries were prepared according to manufactured instruction (SOLID), and miRNAs were sequenced. Data analysis was carried out using CLCbio software. First, raw data were annotated using miRBase and normalized by total reads followed by differential expression analysis. Results validation were performed by RTqPCR in an independent cohort (n= 195). Functional studies of differentially expressed miRNAS were done using in silico tools. Statistical significance was considered at p<0.05. Results: 28 miRNAs were significantly up-regulated and 11 were down...
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-05-20 |