6533b821fe1ef96bd127afdd
RESEARCH PRODUCT
A molecular-based identification resource for the arthropods of Finland
Aino JuslénPetri MetsäläDina SolimanSusana Miranda RomoIka ÖSterbladPetri LampilaTimo PajunenJanne KoskinenErkki M. LaasonenKate PerezSantiago BlancoVladislav IvanovLeif SchulmanLeif SchulmanSami HaapalaAki RinneRitva PenttinenJari-pekka KaitilaJukka SalmelaJostein KjærandsenNadia NikolovaPetri ParkkoCatherine WeiMartti RaekunnasPetri AhlrothPekka RaukkoJuho PaukkunenMikko PentinsaariAri KakkoClaudia SteinkeValerie Levesque-beaudinArto MuinonenTeemu RintalaPetri MartikainenJussi VilenEero J. VesterinenEero J. VesterinenRenee MiskieTomas RoslinTomas RoslinLauri KailaJouni AspiPasi SihvonenRiikka EloEsko ViitanenVeli VikbergGergely VárkonyiChris HoMargarita MiklasevskajaLeena LaasonenRiitta SavolainenJevgeni JakovlevSuresh NaikSampsa MalmbergKari NupponenGunilla StåhlsLiuqiong LuEero HelveMeeri RannistoReijo JussilaDean ChanJari IlmonenSean ProsserJeremy R DewaardMeredith MillerMeri LähteenaroMeri LähteenaroJayme E SonesJaakko PohjoismäkiPekka MajuriTom ClayhillsLauri PaasivirtaSujeevan RatnasinghamPerttu AnttonenConnor P WarneJuha SalokannelJaclyn MckeownVeli-matti MukkalaErkka LaineJuhani ItämiesSami KarjalainenOtso OvaskainenOtso OvaskainenKaj WinqvistPaul D. N. HebertMiduna RahulanTyler ElliotJere KahanpääAli KarhuPanu SomervuoMatti ViitasaariMarko MutanenPetri HirvonenJukka TabellStephanie L. DewaardEvgeny ZakharovMikko TiusanenIiro KakkoGergin BlagoevRamya ManjunathJireh AgdaJaakko Mattilasubject
0106 biological sciencesResource (biology)DNA barcodesspecies identificationBiodiversityreference libraryarthropodsBiologyBarcodeProbabilistic taxonomic assignment010603 evolutionary biology01 natural scienceslaw.inventionCoiCOI03 medical and health sciencessymbols.namesakeProtaxlawprobabilistic taxonomic assignmentniveljalkaisetGeneticsAnimalsDNA Barcoding TaxonomicSpecies identificationArthropodsSpecies identificationFinlandEcology Evolution Behavior and SystematicsGene Library030304 developmental biology0303 health sciencesDNA-analyysiPROTAXsystematiikka (biologia)Linnaean taxonomyReference libraryBiodiversityGeographyEvolutionary biologyDna barcodes1181 Ecology evolutionary biologysymbolsidentificationlajinmääritysIdentification (biology)Biotechnologydescription
Publisher Copyright: © 2021 The Authors. Molecular Ecology Resources published by John Wiley & Sons Ltd. To associate specimens identified by molecular characters to other biological knowledge, we need reference sequences annotated by Linnaean taxonomy. In this study, we (1) report the creation of a comprehensive reference library of DNA barcodes for the arthropods of an entire country (Finland), (2) publish this library, and (3) deliver a new identification tool for insects and spiders, as based on this resource. The reference library contains mtDNA COI barcodes for 11,275 (43%) of 26,437 arthropod species known from Finland, including 10,811 (45%) of 23,956 insect species. To quantify the improvement in identification accuracy enabled by the current reference library, we ran 1000 Finnish insect and spider species through the Barcode of Life Data system (BOLD) identification engine. Of these, 91% were correctly assigned to a unique species when compared to the new reference library alone, 85% were correctly identified when compared to BOLD with the new material included, and 75% with the new material excluded. To capitalize on this resource, we used the new reference material to train a probabilistic taxonomic assignment tool, FinPROTAX, scoring high success. For the full-length barcode region, the accuracy of taxonomic assignments at the level of classes, orders, families, subfamilies, tribes, genera, and species reached 99.9%, 99.9%, 99.8%, 99.7%, 99.4%, 96.8%, and 88.5%, respectively. The FinBOL arthropod reference library and FinPROTAX are available through the Finnish Biodiversity Information Facility (www.laji.fi) at https://laji.fi/en/theme/protax. Overall, the FinBOL investment represents a massive capacity-transfer from the taxonomic community of Finland to all sectors of society. Peer reviewed
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2022-01-01 |