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RESEARCH PRODUCT

Upconversion Nanocarriers Encapsulated with Photoactivatable Ru Complexes for Near-Infrared Light-Regulated Enzyme Activity.

Zhijun ChenSapun H. ParekhRaweewan ThiramanasVolker MailänderVolker MailänderSi WuChaoming XieMischa Schwendy

subject

Materials scienceCell SurvivalInfrared RaysCathepsin KNanoparticle02 engineering and technology010402 general chemistryPhotochemistry01 natural sciencesRutheniumBiomaterialsCell Line TumorLNCaPHumansGeneral Materials ScienceEnzyme Inhibitorsneoplasmschemistry.chemical_classificationbiologytechnology industry and agricultureGeneral ChemistryMesoporous silicaequipment and supplies021001 nanoscience & nanotechnologyPhoton upconversionEnzyme assay0104 chemical sciencesEnzymechemistryEnzyme inhibitorbiology.proteinNanoparticlesNanocarriers0210 nano-technologyBiotechnology

description

Enzyme activity is important for metabolism, cell functions, and treating diseases. However, remote control of enzyme activity in deep tissue remains a challenge. This study demonstrates near-infrared (NIR) light-regulated enzyme activity in living cells based on upconverting nanoparticles (UCNPs) and a photoactivatable Ru complex. The Ru complex is a caged enzyme inhibitor that can be activated by blue light. To prepare a nanocarrier for NIR photoinhibition of enzyme activity, a UCNP and the caged enzyme inhibitors are encapsulated in a hollow mesoporous silica nanoparticle. In such a nanocarrier, the UCNP can harvest NIR light and convert it into blue light, which can activate the caged enzyme inhibitors. This photoactivation process is feasible in deep tissue because of the tissue penetration ability of NIR light. The nanocarrier is compatible to LNCaP, PC3, and SAOS-2 cells, which show high enzyme expression. NIR irradiation induces release of the inhibitors and inhibition of enzyme activity in living cells. NIR light provides high spatiotemporal resolution to regulate enzyme activity in deep tissue.

yearjournalcountryeditionlanguage
2017-03-27Small (Weinheim an der Bergstrasse, Germany)
10.1002/smll.201700997https://pubmed.ncbi.nlm.nih.gov/29024342