6533b822fe1ef96bd127cc42
RESEARCH PRODUCT
ON THE OCCURRENCE OF RESPIRATORY COMPONENTS IN RAT-LIVER NUCLEI.
Thomas E. ConoverThomas E. ConoverGünther SiebertGünther Siebertsubject
Cell NucleusHemeproteinCytochrome bCytochrome cResearchRespiratory chainFlavin groupDNABiologyBiochemistryRatschemistry.chemical_compoundHemoglobinsMetabolismBiochemistrychemistryLiverSpectrophotometrybiology.proteinRespiratory pigmentCytochrome c oxidaseCytochromesRNAHemoglobindescription
Summary 1. Low-temperature spectrophotometric studies have been carried out on rat-liver nuclei isolated by two different procedures. Comparison of nuclei prepared in non-aqueous media with those prepared in high-density sucrose reveals only small quantitative differences. 2. The presence of hemoglobin, cytochrome b 5 , and cytochrome c was detected in both types of nuclei. No cytochrome b , or cytochrome oxidase could be found. Studies on the possible origin of the hemoproteins suggest that hemoglobin and cytochrome b 5 are of extra-nuclear origin. The presence of cytochrome c as a nuclear component could not be ruled out completely although leakage from mitochondria was also considered a possibility. This cytochrome c appears to be metabolically active in isolated systems. The presence of flavin in isolated nuclei of both types was also observed, and the possible extra-nuclear origin considered. 3. It is concluded that a respiratory chain involving hemoprotem components is very unlikely in the nuclei of whole cells. It is possible, however, that the respiratory pigments observed in isolated nuclei may serve as part of an artificial respiratory chain in isolated systems.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1965-04-26 | Biochimica et biophysica acta |