6533b822fe1ef96bd127ccaa

RESEARCH PRODUCT

High-performance liquid chromatography of lactose with evaporative light scattering detection, applied to determine fine particle dose of carrier in dry powder inhalation products

Heribert HäuslerPeter LangguthPeter GrassBianca Beilmann

subject

Detection limitChromatographyLightChemistryHydrophilic interaction chromatographyOrganic ChemistryExtraction (chemistry)Analytical chemistryReproducibility of ResultsLactoseGeneral MedicineBiochemistryHigh-performance liquid chromatographyLight scatteringAnalytical ChemistrySolventChromatography detectorAdministration InhalationScattering RadiationParticlePowdersChromatography High Pressure Liquid

description

A method for quantification of the fine particle dose of lactose is described, using a hydrophilic interaction chromatography (HILIC) method and evaporative light scattering detection. The HILIC method used an aminopropyl column and a mobile phase consisting of acetonitril/water (80/20, v/v) for isocratic elution. Sensitive chromatography was obtained using a low concentration of water in the extraction solvent. The detection limit (RSD10%) at an injection volume of 10 microL is 10 microg/mL. Linearity was obtained in the range of 10-80 microg/mL (R(2)0.99). A relative standard deviation (RSD) of 0.5% (N=6) demonstrated good precision of the optimized method.

yearjournalcountryeditionlanguage
2005-08-10Journal of Chromatography A
https://doi.org/10.1016/j.chroma.2005.12.083