6533b822fe1ef96bd127ce90
RESEARCH PRODUCT
Topology of the 10 subunits within the decamer of KLH, the hemocyanin of the marine gastropod Megathura crenulata.
Jürgen MarklJ. Robin HarrisWolfgang Gebauersubject
Peanut agglutininGene isoformModels MolecularImmunoelectron microscopymedicine.medical_treatmentProtein subunitchemical and pharmacologic phenomenaHemocyaninBiologyMegathura crenulatabiology.organism_classificationCrystallography X-RayMolecular biologyNegative stainMolecular WeightMicroscopy ElectronProtein SubunitsStructural BiologyMolluscaHemocyaninsmedicinebiology.proteinAnimalsProtein Structure QuaternaryKeyhole limpet hemocyanindescription
Immunoelectron microscopy has been performed using negatively stained immune complexes of keyhole limpet hemocyanin isoform 1 (KLH1) decamers and a functional unit-specific monoclonal antibody anti-KLH1-c1. The antibody links hemocyanin molecules at both the collar and the collarless edge of the decamer, indicating a peripheral localization of functional units c. In isoform 2 (KLH2) the positions of functional units c have been identified with the peanut agglutinin (PNA), which has previously been shown to exclusively bind to KLH2-c. Ferritin linked to PNA was used to visualize labeled molecules electron microscopically. The pattern of labeling also indicates a peripheral localization of the c functional units. The data presented in this paper support only one of two possible models for the subunit orientation within the hemocyanin decamer.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2002-09-01 | Journal of structural biology |