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RESEARCH PRODUCT

Polarization microscopy as a tool for quantitative evaluation of collagen using picrosirius red in different stages of CKD in cats

J. M. G. VerdugoCélia Maria De Souza SampaioArnaldo AlvesDa VianaC. D'ó PessoaMário Rogério Lima MotaJoão Alison De Moraes SilveiraJ. S. A. M. EvangelistaF. M. O. SilvaF. A. F. Xavier JúniorGlayciane Bezerra De MoraisKaio Moraes De FariasFerraz Da Silva

subject

Male0301 basic medicinePathologymedicine.medical_specialtyHistology040301 veterinary sciencesCat DiseasesKidneySeverity of Illness IndexPicrosirius red0403 veterinary science03 medical and health scienceschemistry.chemical_compoundDisease severityTrichromeFibrosismedicineAnimalsRenal Insufficiency ChronicColoring AgentsInstrumentationCreatinineKidneyCATSbusiness.industry04 agricultural and veterinary sciencesmedicine.diseaseFibrosisMedical Laboratory Technology030104 developmental biologymedicine.anatomical_structurechemistryCreatinineCatsFemaleCollagenMicroscopy PolarizationAnatomybusinessAzo CompoundsKidney disease

description

Chronic kidney disease (CKD) is a relevant disease in feline clinic. The tubulointerstitial damage, with collagen deposition and fibrosis, is an important result of this process. The aim of this study was to quantify and correlate the deposition of collagen and severity of interstitial fibrosis (IF) in the kidney from cats in different stages of CKD. Kidney fragments from 10 adult cats with CKD were analyzed and stained by Masson's trichrome (MT) and Picrosirius red (PSR) for circular polarized microscopy. Random quantitative analysis was performed on MT sections to classify the degree of IF, per field area, with and without circular polarization. Statistics correlations were performed by Spearman's (ρ; p < .05). There was a significant correlation of IF quantification with the area of interstitial collagen deposition by polarized PSR (PSRp) (r = .7939, p = .0098) and nonpolarized PSR (PSRn) (r = .7781, p = .0080). There was a positive correlation of serum creatinine (sCr) at different stages of CKD with PSRp (r = .7939, p = .0098), PSRn (r = .8667, p = .0027) and MT (r = .7818, p = .0117). Correlations between the percentage of quantified area was also positive from PSRp to PSRn (r = .9030, p = .0009) and PSRp to MT (r = .7939, p = .0098). The PSRN was also correlated with MT (r = .9273, p = .0001). The correlation with IF and sCr follows the disease evolution and the quantification of collagen by PSR is an excellent tool for analyzing the disease severity at different stages.

yearjournalcountryeditionlanguage
2016-09-09Microscopy Research and Technique
https://doi.org/10.1002/jemt.22830