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RESEARCH PRODUCT

IMPROVEMENT IN REDOX HOMEOSTASIS AFTER CYTOREDUCTIVE SURGERY IN COLORECTAL ADENOCARCINOMA

Soghra RabizadehGiovanni TomaselloNiloofar NajafiAlireza EsteghamatiManouchehr NakhjavaniArmin RajabAbbas AlibakhshiHossein MirmiranpourSalome Sadat Salehi

subject

Glycation End Products AdvancedMale0301 basic medicineAgingAntioxidantColorectal cancermedicine.medical_treatmentmedicine.disease_causeBiochemistryGastroenterologychemistry.chemical_compoundPostoperative Complications0302 clinical medicineMalondialdehydeHomeostasischemistry.chemical_classificationbiologyGlutathione peroxidaseCytoreduction Surgical ProceduresGeneral MedicineMiddle AgedCatalaseMalondialdehydecolorectal cancer colorectal surgery cytoreductive surgery redox homeostasisLipoproteins LDLCatalase030220 oncology & carcinogenesisFemaleColorectal NeoplasmsResearch ArticleAdultmedicine.medical_specialtyArticle SubjectAdenocarcinomaSuperoxide dismutase03 medical and health sciencesInternal medicinemedicineHumansAgedGlutathione PeroxidaseQH573-671business.industryCell Biologymedicine.diseaseOxidative Stress030104 developmental biologychemistrybiology.proteinCytologybusinessOxidative stressLipoprotein

description

Colorectal cancer (CRC) as one the most common cancer type is associated with oxidative stress. Surgery is the only curative modality for early-stage CRC. The aim of this study was to evaluate the oxidative damage biomarkers as well as enzymatic and nonenzymatic antioxidants in patients with CRC before and after tumor resection and in healthy controls. 60 patients with stage I/II colorectal adenocarcinoma and 43 healthy controls were recruited in this study. We measured plasma levels of oxidative damage biomarkers, including advanced oxidation protein products (AOPP), advanced glycation end products (AGEs), malondialdehyde (MDA), and oxidized low-density lipoprotein (ox-LDL) at baseline and after tumor removal. We also evaluated the plasma activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) as enzymatic antioxidants and the ferric reducing antioxidant power (FRAP) assay for nonenzymatic antioxidant capacity. Patients with CRC had significantly higher AGE, AOPP, MDA, and ox-LDL and also FRAP levels and higher SOD and GPx and lower CAT activity levels compared to healthy controls (p<0.05). We did not observe any statistically significant correlation between redox biomarkers and the size and stage of the tumor. AGEs (72.49±4.7vs.67.93±8.8,p<0.001), AOPP (137.64±21.9vs.119.08±33.1,p<0.001), MDA (3.56±0.30vs.3.05±0.33,p<0.001), and ox-LDL (19.78±0.97vs.16.94±1.02,p<0.001) concentrations reduced significantly after tumor removal. The largest effect sizes were found in ox-LDL (d=−2.853, 95% CI 2.50-3.19) and MDA (d=−1.617, 95% CI 0.43-0.57). Serum FRAP levels (1097.5±156.7vs.1239.3±290,p<0.001) and CAT (2.34±0.34vs.2.63±0.38,p<0.001), GPx (102.37±6.58vs.108.03±6.95,p<0.001), and SOD (5.13±0.39vs.5.53±0.31,p<0.001) activity levels increased significantly after surgery. The largest effect sizes among antioxidants were seen in SOD (d=1.135, 95% CI 0.46-0.34) and GPx (d=0.836, 95% CI 0.35-0.23). This study indicated that patients with colorectal cancer had higher levels of oxidative stress and antioxidant activity compared to healthy controls. After surgical resection of tumor, we observed a substantial improvement in redox homeostasis.

yearjournalcountryeditionlanguage
2021-01-01
10.1155/2021/8864905http://hdl.handle.net/10447/525719