6533b823fe1ef96bd127e211
RESEARCH PRODUCT
Secretion of neutral and acid DNases in cultivated human lymphocytes after incubation with DNA; possible consequences for inhalation anesthesia.
Peter P. KleemannGünter LöberReitz MWolfgang Dicksubject
Time FactorsBiologyGeneral Biochemistry Genetics and Molecular Biologychemistry.chemical_compoundPrecursor cellmedicineDeoxyribonuclease IHumansSecretionLymphocytesIncubationCells CulturedDeoxyribonucleasesEndodeoxyribonucleasesInhalationDose-Response Relationship DrugDNAHydrogen-Ion ConcentrationDose–response relationshipKineticschemistryBiochemistryAnestheticHalothaneAnesthesia InhalationHalothaneDNAmedicine.drugdescription
Abstract After incubation with DNA human lymphocytes release neutral and acid DNase activities into the culture medium; the release depends on DNA concentration and time of cultivation. The electrophoretic mobility of the released neutral DNase activity is in accordance with DNase I and the electrophoretic mobility of the released acid DNase activity with DNase II. The released DNase activities do not originate from dead cells and are not influenced by blast cell formation. The anesthetic halothane can inhibit the released neutral and acid DNase activities. Inhalation anesthesia can possibly disturb the correlation between DNA and DNases in human blood.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1995-06-01 | Zeitschrift fur Naturforschung. C, Journal of biosciences |