H-Point Curve Isolation Method for Coupled Liquid Chromatography and UV−Visible Spectrophotometry

6533b823fe1ef96bd127ec0e

RESEARCH PRODUCT

H-Point Curve Isolation Method for Coupled Liquid Chromatography and UV−Visible Spectrophotometry

Francisco Bosch-reig Carmen Molins-legua Pilar Campíns-falcó F. Blasco-gómez Rosa Herráez-hernández

subject

Analyte Chromatography medicine.diagnostic_test Chemistry Amphetamines Analytical chemistry Analytical Chemistry Chromatography detector Spectrophotometry medicine Spectrophotometry Ultraviolet Diuretics Chromatography column Chromatography Liquid

description

The H-point curve isolation method (HPCIM) for the detection of unknown interferences in chromatography is proposed. The method allows one to estimate the UV-vis spectra of interfering species in a sample as well as to test the purity of the chromatographic peaks. Besides the detection of the unknown interferences in a sample, this method allows one to calculate the concentration of an analyte in the presence of unknown compounds. To illustrate the reliability of the proposed method, samples of diuretics and amphetamines have been analyzed by normal- and reversed-phase high-performance chromatography.

year	journal	country	edition	language
2000-06-17	Analytical Chemistry

https://doi.org/10.1021/ac990649q

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