6533b823fe1ef96bd127ed57

RESEARCH PRODUCT

Characterization of poly(4-vinylpyridine 1-oxide) by free-solution capillary electrophoresis and micellar electrokinetic chromatography

Guillermo Ramis-ramosMiriam Beneito-cambraJosé Manuel Herrero-martínez

subject

AnionsDetergentsClinical BiochemistryOxidemacromolecular substancesBuffersBiochemistryMicelleMicellar electrokinetic chromatographyAnalytical ChemistrySurface tensionchemistry.chemical_compoundCapillary electrophoresisAdsorptionSurface TensionMicellesLaunderingchemistry.chemical_classificationChromatographyChromatographytechnology industry and agricultureElectrophoresis CapillaryWaterPolymerPhosphateOrganophosphatesSolutionschemistryPolyvinylpyridine N-Oxide

description

The migration characteristics of poly(4-vinylpyridine 1-oxide) (PVP-NO) in phosphate buffers of acidic pH (20 mM H 3 PO 4 or NaH 2 PO 4 ) have been studied using both free-solution capillary electrophoresis (FSCE) and MEKC. To inhibit adsorption, 250 mM o-phosphoethanolamine (2-aminoethyl dihydrogen phosphate) was used. In FSCE, PVP-NO showed a narrow peak and a broader band, both having anionic behavior. These peak and band were attributed to the free and aggregated or micellized PVP-NO forms, respectively. According to surface tension measurements, the CMC of SDS in the BGE was 1.8 and 0.48 mM in the absence and in the presence of 1000 μpg/mL PVP-NO, respectively, and the association of the polymer with SDS was completed at 9.7 mM SDS. Using MEKC, a narrow peak and a broader band also appeared at SDS concentrations of ca. 1 mM, and their intensity increased with the SDS concentration. These peak and band were attributed to the formation of mixed micelles constituted by both free PVP-NO/SDS and aggregated PVP-NO/SDS, respectively. The determination of PVP-NO by FSCE in commercial additives for laundry was demonstrated.

yearjournalcountryeditionlanguage
2008-06-27ELECTROPHORESIS
https://doi.org/10.1002/elps.200800118