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RESEARCH PRODUCT
Salivary MMP-9 in the detection of oral squamous cell carcinoma
Andre PeiskerArndt GuentschGregor F. RaschkeJoschka HenningsMina D. FahmyKorosh RoshanghiasStefan Schultze-mosgausubject
AdultMaleSalivamedicine.medical_specialtyPathologyMatrix metalloproteinaseGastroenterology03 medical and health sciences0302 clinical medicineInterquartile rangeInternal medicineBiomarkers TumorCarcinomaHumansMedicineProspective StudiesSalivaProspective cohort studyGeneral DentistryAgedAged 80 and overOral Medicine and PathologyReceiver operating characteristicbusiness.industryResearchCancer030206 dentistryMiddle Agedmedicine.disease:CIENCIAS MÉDICAS [UNESCO]stomatognathic diseasesMatrix Metalloproteinase 9OtorhinolaryngologySalivary diagnostics030220 oncology & carcinogenesisUNESCO::CIENCIAS MÉDICASCarcinoma Squamous CellFemaleMouth NeoplasmsSurgerybusinessdescription
Background Oral squamous cell carcinoma (OSCC) is the most common malignant tumour of the oral cavity. Detection of OSCC is currently based on clinical oral examination combined with histopathological evaluation of a biopsy sample. Direct contact between saliva and the oral cancer makes measurement of salivary metalloproteinase- 9 (MMP-9) an attractive alternative. Material and Methods In total, 30 OSCC patients and 30 healthy controls were included in this prospective study. Saliva samples from both groups were collected, centrifuged and supernatant fluid was subjected to ELISA for assessment of MMP-9. The median salivary MMP-9 values with interquartile range (IQR) of OSCC patients and the control group were statistically analysed using the Mann-Whitney U-test. The receiver operating characteristic (ROC) curve was constructed and the area under curve (AUC) was computed. Results The median absorbance MMP-9 value of the OSCC group was 0.186 (IQR=0.158) and that of control group was 0.156 (IQR=0.102). MMP-9 was significantly increased in the OSCC patients than in the controls by +19.2% (p=0.008). Median values in patients with recurrence and in patients with primary event were 0.233 (IQR=0.299) and 0.186 (IQR=0.134) respectively. MMP-9 was significantly increased in patients with primary event (p=0.017) compared to controls by +19.2%. No significant increase of MMP-9 level was detected when comparing patients with recurrence and healthy controls (+49.4%; p=0.074). The sensitivity value of MMP-9 was 100% whereas the specificity value was 26.7% with AUC of 0.698. Conclusions The present data indicates that the elevation of salivary levels of MMP-9 may be a useful adjunctive diagnostic tool for detection of OSCC. However, further studies are necessary to provide scientific and clinical validation. Key words:Oral squamous cell carcinoma, oral cancer, saliva, salivary diagnostics, cancer detection, MMP-9, metalloproteinases.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-02-01 |