6533b824fe1ef96bd1280047

RESEARCH PRODUCT

Mosaic Qβ coats as a new presentation model

Velta OsePaul PumpensInta VasiljevaIndulis CielensAnna StrelnikovaAndris KazaksTatjana Kozlovska

subject

AntigenicityRecombinant Fusion ProteinsGenetic VectorsBiophysicsBiologyHepatitis b surface antigenBiochemistryEpitopelaw.inventionCapsid assemblyMiceCapsidPhage QβPeptide LibraryStructural BiologylawGeneticsAnimalsHepatitis B virus preS1Cloning MolecularMolecular BiologyAllolevivirusMice Inbred BALB CCoat protein UGA suppressionVirus AssemblyImmunogenicityA1 extensionRNACell BiologyImmunogenicityVirologyMolecular biologyCapsidCarrier proteinCodon TerminatorRecombinant DNACapsid Proteins

description

The new protein carrier was developed on the basis of recombinant RNA phage Qbeta capsid. C-terminal UGA extension of the short form of Qbeta coat, so-called A1 extension, served as a target for presentation of foreign peptides on the outer surface of mosaic Qbeta particles. In conditions of enhanced UGA suppression, the proportion of A1-extended to short coats in mosaic particles dropped from 48% to 14%, with an increase of the length of A1 extension. A model insertion, short preS1 epitope 31-DPAFR-35 of hepatitis B surface antigen, demonstrated superficial location on the mosaic Qbeta particles and ensured specific antigenicity and immunogenicity.

yearjournalcountryeditionlanguage
1998-07-10FEBS Letters
https://doi.org/10.1016/s0014-5793(98)00716-9