6533b824fe1ef96bd1280cc8
RESEARCH PRODUCT
Application of the 6-aminoquinolyl-N-hydroxysccinimidyl carbamate (AQC) reagent to the RP-HPLC determination of amino acids in infant foods.
Amparo AlegríaLourdes BoschRosaura Farrésubject
Carbamatemedicine.medical_treatmentClinical BiochemistryBiochemistryAnalytical Chemistrychemistry.chemical_compoundmedicineHumansProlineAmino AcidsDerivatizationChromatography High Pressure Liquidchemistry.chemical_classificationDetection limitChromatographyChemistryInfantReproducibility of ResultsCell BiologyGeneral MedicineAmino acidReagentAminoquinolinesIndicators and ReagentsInfant FoodCarbamatesLeucineEdible GrainCysteinedescription
The validation of a pre-column derivatization procedure with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) to the determination of the amino acid content by RP-HPLC with fluorescence detection (lambda excitation 250 nm, lambda emission 395 nm) in milk-cereal based infant foods was carried out. The analytical parameters: linearity (0.0025-0.2mM), precision of the method (0.2-3.5% variation coefficients), accuracy (derivatization: 86-106% average recovery and method: 88.3-118.2% average recovery) and the limits of detection (0.016-0.367 microM) and quantification (0.044-1.073 microM) were determined. Glutamic acid, proline and leucine were the most abundant amino acid whereas the lowest contents corresponded to tyrosine and cysteine.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2005-02-21 | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |