6533b824fe1ef96bd1281507
RESEARCH PRODUCT
Reactive Self-Assembly and Specific Cellular Delivery of NCO-sP(EO-stat-PO)-Derived Nanogels
Outi PaloheimoKrystyna AlbrechtMartin MöllerJürgen GrollElina MäntyläHaika HildebrandtMaija Vihinen-rantaSatu HakanenSami F. Willmansubject
Polymers and Plasticsta221Bioengineering02 engineering and technology010402 general chemistry01 natural sciencesPolyethylene GlycolsBiokemia solu- ja molekyylibiologia - Biochemistry cell and molecular biologyBiomaterialschemistry.chemical_compoundnanogelsDrug Delivery SystemsAmphiphileMaterials ChemistryHumansPolyethyleneimineMoleculeMoietynanopolymeeritreactive self-assemblyPeptide sequenceFluorescent DyesIsophoronegeelitta1182nanobiotekniikka021001 nanoscience & nanotechnologyIsocyanate0104 chemical scienceschemistryCovalent bondBiophysicsNanoparticlesSelf-assembly0210 nano-technologyHeLa CellsBiotechnologydescription
This study presents the reactive self-assembly of isocyanate functional and amphiphilic six-arm, star-shaped polyether prepolymers in water into nanogels. Intrinsic molecular amphiphilicity, mainly driven by the isophorone moiety at the distal endings of the star-shaped molecules, allows for the preparation of spherical particles with an adjustable size of 100-200 nm by self-assembly and subsequent covalent cross-linking without the need for organic solvents or surfactants. Covalent attachment of a fluorescence dye and either the cell-penetrating TAT peptide or a random control peptide sequence shows that only TAT-labeled nanogels are internalized by HeLa cells. The nanogels thus specifically enter the cells and accumulate in the perinuclear area in a time- and concentration-dependent manner.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-01-01 |