Fermentācijas apstākļu optimizācija inulīna konversijai bioetanolā, izmantojot raugu Kluyveromyces marxianus

6533b825fe1ef96bd128225b

RESEARCH PRODUCT

Fermentācijas apstākļu optimizācija inulīna konversijai bioetanolā, izmantojot raugu Kluyveromyces marxianus

subject

inulīns fermentācija bioetanols topinambūrs Kluyveromyces marxianus Bioloģija

description

Darba mērķis bija apskatīt dažādu fermentācijas apstākļu ietekmi uz inulīna konversiju bioetanolā ar raugu Kluyveromyces marxianus. Pētījumā tika salīdzināti divi K.marxianus celmi DSM 5418 un DSM 5422. Celms K. marxianus DSM 5422 uzrādīja labākus augšanas un etanola produktivitātes rādītājus inulīnu saturošās barotnēs. Tika veikti daudzfaktoru eksperimenti, kuros aplūkota fermentācijas temperatūras, substrāta koncentrācijas un aerācijas ietekme uz etanola un biomasas biosintēzes rādītājiem inulīna barotnēs. Novērtēta minēto faktoru savstarpējā iedarbība. Konstatēts, ka visoptimālākie apstākļi etanola producēšanai no inulīna ar K.marxianus DSM 5422 ir pie 30°C temperatūras, 0,2 l•l-1•min-1 aerācijas un inulīna barotnes koncentrācijas 150 g/l. Šī darba veikšanai tika apgūtas sekojošas eksperimentālās metodes: kopējo cukuru noteikšana, glikozes un fruktozes noteikšana enzimātiski, K.marxianus kultivēšana, fermentācija un paraugu sagatavošana etanola noteikšanai ar HPLC. Pētījumi tika veikti LU Mikrobioloģijas un biotehnoloģijas institūtā, Ogļhidrātu biokonversijas laboratorijā, laika posmā no 2014.gada septembra līdz 2015.gada februārim.

year	journal	country	edition	language
2015-01-01

https://dspace.lu.lv/dspace/handle/7/28550

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