Multilocus microsatellite analysis of European and African Candida glabrata isolates

6533b825fe1ef96bd1282834

RESEARCH PRODUCT

Multilocus microsatellite analysis of European and African Candida glabrata isolates

Burcu Dalyan Cilo Nnaemeka Emmanuel Nnadi Fabio Scordino V. Chillemi H.-j. Tietz C. Lo Passo Demetrio Delfino Antonio Cascio Paula Sampaio Giuseppe Criseo S. Rharmitt A.d. Van Diepeningen Javier Pemán Orazio Romeo

subject

0301 basic medicine Settore MED/07 - Microbiologia E Microbiologia Clinica Clonal complex Epidemiology Multilocus microsatellite analysis Candida glabrata Molecular phylogeny Gene locus Central type Related genotype Genotype Environmental Microbiology Haplotype DNA Fungal Priority journal Genetics Allele Candidiasis General Medicine Classification Europe Phenotype Infectious Diseases Candida Glabrata; Adhesins; Fluconazole Candidiasi Microsatellite Microsatellite Repeat Microbiological examination Human Microbiology (medical)Genotype Settore MED/17 - Malattie Infettive Microsatellite DNA 030106 microbiology Biology European Microbiology Article 03 medical and health sciences Genetic variation Microsatellite repeats Genetics Humans Allele Genotyping Alleles Science & Technology Candida glabrata Microsatellite marker Haplotype African Genetic Variation biology.organism_classification Nonhuman 030104 developmental biology Fungal DNA Haplotypes Isolation and purification Genetic Loci Africa Multilocus sequence typing Fungus isolation Genetic variability Microsatellite genotype Multilocus Sequence Typing

description

This study aimed to elucidate the genetic relatedness and epidemiology of 127 clinical and environmental Candida glabrata isolates from Europe and Africa using multilocus microsatellite analysis. Each isolate was first identified using phenotypic and molecular methods and subsequently, six unlinked microsatellite loci were analyzed using automated fluorescent genotyping. Genetic relationships were estimated using the minimum-spanning tree (MStree) method. Microsatellite analyses revealed the existence of 47 different genotypes. The fungal population showed an irregular distribution owing to the over-representation of genetically different infectious haplotypes. The most common genotype was MG-9, which was frequently found in both European and African isolates. In conclusion, the data reported here emphasize the role of specific C. glabrata genotypes in human infections for at least some decades and highlight the widespread distribution of some isolates, which seem to be more able to cause disease than others.

year	journal	country	edition	language
2016-06-01

10.1007/s10096-016-2610-3