6533b825fe1ef96bd128321d

RESEARCH PRODUCT

Use of the modified quick easy cheap effective rugged and safe sample preparation approach for the simultaneous analysis of type A- and B-trichothecenes in wheat flour

José Miguel SorianoIsabel SospedraJordi MañesJesús Blesa

subject

AcetonitrilesChromatographyChemistryMethanolFlourOrganic ChemistryExtraction (chemistry)Wheat flourAnalytic Sample Preparation MethodsGeneral MedicineRepeatabilityQuechersBiochemistryMass SpectrometryDiacetoxyscirpenolAnalytical Chemistrychemistry.chemical_compoundVomitoxinLiquid chromatography–mass spectrometrySolventsSample preparationTrichothecenesTriticumChromatography Liquid

description

A suitable extraction and purification method for the simultaneous liquid chromatography-mass spectrometry (LC-MS) determination of five mycotoxins, three type A, diacetoxyscirpenol (DAS), T-2 toxin (T-2) and HT-2 toxin (HT-2), and two type B-trichothecenes, deoxynivalenol (DON) and nivalenol (NIV), has been optimised using a modified "Quick Easy Cheap Effective Rugged and Safe" (QuEChERS) method. Different solvents were studied in the extraction procedure to obtain better recoveries, which ranged from 86 to 108%, using a 85/15 (v/v) mixture of methanol/acetonitrile. The values obtained for recovery, repeatability and reproducibility of the optimized method are in agreement with Commission Directive 2005/26/EC for methods of analysis of Fusarium toxins. Finally, this optimized procedure was applied in wheat flour samples commercialized in Spain.

yearjournalcountryeditionlanguage
2010-02-01Journal of Chromatography A
https://doi.org/10.1016/j.chroma.2009.12.047