6533b826fe1ef96bd1283f31

RESEARCH PRODUCT

Isolation and characterization of 11 polymorphic trinucleotide microsatellite markers in the stonefly Arcynopteryx compacta (Plecoptera: Perlodidae).

Steffen U. PaulsKevin A. FeldheimKathrin TheissingerAlfred Seitz

subject

Geneticseducation.field_of_studybiologyPopulationbiology.organism_classificationNull alleleLoss of heterozygosityPerlodidaeGenetic structureGeneticsMicrosatelliteAlleleeducationMicrosatellite enrichmentEcology Evolution Behavior and SystematicsBiotechnology

description

We describe the isolation of 11 polymorphic trinucleotide microsatellite loci from the stonefly Arcynopteryx compacta. Loci were highly variable with 3 to 14 alleles (mean = 6.45). Observed heterozygosity ranged from 0 to 0.867. Seven loci showed significant deviation from Hardy–Weinberg equilibrium across both populations. There was no evidence for null alleles, and thus, Hardy–Weinberg departures could have resulted from genetic structure between populations or subpopulations. No linkage between loci was found. The 11 loci should prove highly informative for population genetic studies.

10.1111/j.1755-0998.2008.02450.xhttps://pubmed.ncbi.nlm.nih.gov/21564649