Glacial survival and post-glacial recolonization of an arctic-alpine freshwater insect (Arcynopteryx dichroa, Plecoptera, Perlodidae) in Europe
Aim General models for understanding the climate-driven processes of post-glacial colonization in European arctic–alpine species are mainly derived from studies on temperate terrestrial taxa. However, cold-adapted freshwater species may tolerate or even thrive under colder climatic conditions as flowing water habitats are thermally buffered against freezing and extremely cold temperatures. Here, we investigate the European Pleistocene and Holocene history of the arctic–alpine stonefly Arcynopteryx dichroa. Location Europe. Methods We used two genetic data sets (mitochondrial sequence data and nuclear microsatellite data) to investigate the glacial survival and post-glacial recolonization ro…
Cryptic biodiversity loss linked to global climate change
Climate impacts on biodiversity are usually assessed at the morphospecies level. An analysis of the distribution and mitochondrial DNA variability of nine montane aquatic insect species in Europe suggests range contractions will be accompanied by severe loss of genetic diversity. These results imply that morphospecies-scale assessments may greatly underestimate potential biodiversity losses from climate change.
Isolation and characterization of 11 polymorphic trinucleotide microsatellite markers in the stonefly Arcynopteryx compacta (Plecoptera: Perlodidae).
We describe the isolation of 11 polymorphic trinucleotide microsatellite loci from the stonefly Arcynopteryx compacta. Loci were highly variable with 3 to 14 alleles (mean = 6.45). Observed heterozygosity ranged from 0 to 0.867. Seven loci showed significant deviation from Hardy–Weinberg equilibrium across both populations. There was no evidence for null alleles, and thus, Hardy–Weinberg departures could have resulted from genetic structure between populations or subpopulations. No linkage between loci was found. The 11 loci should prove highly informative for population genetic studies.
The era of reference genomes in conservation genomics
Progress in genome sequencing now enables the large-scale generation of reference genomes. Various international initiatives aim to generate reference genomes representing global biodiversity. These genomes provide unique insights into genomic diversity and architecture, thereby enabling comprehensive analyses of population and functional genomics, and are expected to revolutionize conservation genomics.
Isolation and characterization of 10 highly polymorphic di- and trinucleotide microsatellite markers in the mayfly Ameletus inopinatus (Ephemeroptera: Siphlonuridae).
We describe the isolation of ten polymorphic microsatellite loci from the mayfly Ameletus inopinatus. Loci had di- or trinucleotide repeat motifs and were highly variable with three to 17 alleles (mean = 7.15). Observed heterozygosity ranged from 0.143 to 0.905. One locus (Ami_202) showed significant deviation from Hardy–Weinberg equilibrium in one population, but no evidence for null alleles. One locus (Ami_73) was significantly linked with three other loci. The remaining nine loci should prove highly informative for population genetic studies.
Molecular data and species distribution models reveal the Pleistocene history of the mayfly Ameletus inopinatus (Ephemeroptera: Siphlonuridae)1
SUMMARY 1. We investigated the Pleistocene and Holocene history of the rare mayfly Ameletus inopinatus EATON 1887 (Ephemeroptera: Siphlonuridae) in Europe. We used A. inopinatus as a model species to explore the phylogeography of montane, cold-tolerant aquatic insects with arctic–alpine distributions. 2. Using species distribution models, we developed hypotheses about the species demographic history in Central Europe and the recolonisation history of Fennoscandia. We tested these hypotheses using mitochondrial cytochrome oxidase I (mtCOI) sequence data and compared our genetic results with previously generated microsatellite data to explore genetic diversity distributions of A. inopinatus. …
The Carpathians as a Major Diversity Hotspot in Europe
The Carpathians are one of the major mountain ranges of Europe, but still one of its least studied regions. It is increasingly recognized that they played a major role in the formation and Pleistocene survival of numerous continental, arctic, and arctic–alpine taxa. Many endemic taxa have been described from these mountains. The number of phylogeographic/phylogenetic studies covering at least partially the Carpathians is also increasing. These studies reveal unevenly distributed genetic and taxonomic diversity. In this work, we analyse population genetic structures in the Carpathians revealed by case studies on aquatic insects, comparing them to existing literature data on plants, butterfli…