6533b834fe1ef96bd129d66c

RESEARCH PRODUCT

Isolation and characterization of 10 highly polymorphic di- and trinucleotide microsatellite markers in the mayfly Ameletus inopinatus (Ephemeroptera: Siphlonuridae).

Kathrin TheissingerSteffen U. PaulsJulia TaubmannAlfred SeitzKevin A. Feldheim

subject

Geneticseducation.field_of_studyPopulationLocus (genetics)Biologybiology.organism_classificationNull alleleLoss of heterozygosityMayflyGeneticsMicrosatelliteeducationTrinucleotide repeat expansionMicrosatellite enrichmentEcology Evolution Behavior and SystematicsBiotechnology

description

We describe the isolation of ten polymorphic microsatellite loci from the mayfly Ameletus inopinatus. Loci had di- or trinucleotide repeat motifs and were highly variable with three to 17 alleles (mean = 7.15). Observed heterozygosity ranged from 0.143 to 0.905. One locus (Ami_202) showed significant deviation from Hardy–Weinberg equilibrium in one population, but no evidence for null alleles. One locus (Ami_73) was significantly linked with three other loci. The remaining nine loci should prove highly informative for population genetic studies.

yearjournalcountryeditionlanguage
2008-11-01Molecular ecology resources
10.1111/j.1755-0998.2008.02356.xhttps://pubmed.ncbi.nlm.nih.gov/21586022