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RESEARCH PRODUCT
Sleeping Beauty transposon system – future trend in T-cell-based gene therapies?
Florian RohrbachMatthias TheobaldRuth Frommoltsubject
GeneticsTransposable elementCancer ResearchT-LymphocytesT cellGenetic enhancementGene Transfer TechniquesTransposasesGenetic TherapyGeneral MedicineTransfectionBiologyTransfectionSleeping Beauty transposon systembiology.organism_classificationTransduction (genetics)medicine.anatomical_structureRetrovirusOncologymedicineHumansTransgenesGenedescription
Evaluation of: Huang X, Wilber AC, Bao L et al.: Stable gene transfer and expression in human primary T cells by the Sleeping Beauty transposon system. Blood 107, 483–491 (2006). The Sleeping Beauty (SB) transposon system can mediate stable gene transfer and expression in primary human T cells. Optimal in vitro conditions for maximum gene transfer efficiencies have been developed with regard to further application of the SB transposon system in T cell based gene therapies. This raises the question of whether or not the SB transposon system is a convincing alternative for virus-mediated gene transfer based on the currently available data. Here, we will discuss controversial safety and efficacy issues in transposon and viral gene transfer technology.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-06-01 | Future Oncology |