6533b827fe1ef96bd1285b53
RESEARCH PRODUCT
Applicability of the isolated perfused rat brain for studying central cholinergic mechanisms.
Josef KrieglsteinHeinz Kilbingersubject
MalePhysostigmineChromatography GasPhysostigmineEndogenyPharmacologyElectroencephalographyIn Vitro TechniquesIn vivomedicineOxotremorineAnimalsPharmacologyBrain Chemistrymedicine.diagnostic_testChemistryOxotremorineElectroencephalographyGeneral MedicineIsolated brainAcetylcholineStimulation ChemicalRatsPerfusionPerfusionAcetylcholinemedicine.drugdescription
The ACh content of the isolated perfused rat brain as well as that of the rat brain in vivo was determined by gas chromatography. After a perfusion period of 30 min the endogenous ACh content of the isolated brain was significantly higher than that of the brain in vivo. Physostigmine caused a rise in the ACh concentration of both the isolated brain and the brain in vivo. Oxotremorine produced an increase in the ACh content in the brain in vivo, but not in the isolated brain after appropriate dosage. The concentration of the drugs in the perfusion medium of the isolated brain was so that distinct EEG changes could be observed but spontaneous electrical activity could be maintained during the whole perfusion period.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1974-01-01 | Naunyn-Schmiedeberg's archives of pharmacology |