6533b828fe1ef96bd128798b
RESEARCH PRODUCT
Hemocyanin subunit organization of the gastropod Rapana thomasiana
Benedetto SalvatoJürgen MarklEnrico DaineseWolfgang VoelterMariano BeltraminiStanka StoevaWolfgang Gebauersubject
Gene isoformSubunitProtein subunitmedicine.medical_treatmentMolecular Sequence DataBiophysicsImmunoelectrophoresisBiologyMegathura crenulataCleavage (embryo)BiochemistryHigh-performance liquid chromatographyHemocyaninRapana thomasianamedicineAnimalsProtein IsoformsAmino Acid SequenceProtein Structure QuaternaryMolecular BiologyGasteropodsmedicine.diagnostic_testPancreatic ElastaseImmunochemistryStructureHemocyaninbiology.organism_classificationMolecular biologyPeptide FragmentsMolluscaHemocyanin; Gasteropods; Structure; SubunitHemocyaninsImmunoelectrophoresis Two-Dimensionaldescription
Abstract RtH1 and RtH2, the two hemocyanin isoforms of the prosobranch gastropod Rapana thomasiana, have been purified by anion-exchange chromatography and studied by SDS–PAGE and immunoelectrophoresis. Both subunit types are built up of eight functional units (FUs). Under reducing conditions subunit RtH2 splits into two fragments, RtH2- a – f and RtH2- gh, suggesting the presence of a disulfide bridge between FU2- f and FU2- g. By proteolytic cleavage of the subunits into three-, two-, and single-FU fragments, purification of fragments by HPLC, N-terminal sequencing of the peptides, and crossed-line immunoelectrophoresis, FUs- a – h of RtH2 and FU- a, FU- d, FU- e, and FU- f of RtH1 were identified and correlated to the eight-FUs pattern of immunoelectrophoresis. FU- a, FU- e, and FU- f of RtH1 and RtH2 are very closely related immunologically. RtH1 and RtH2 both correspond immunologically to KLH2, one of the two hemocyanin isoforms of the prosobranch gastropod Megathura crenulata.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1999-01-01 |