6533b828fe1ef96bd1288e7a

RESEARCH PRODUCT

Detection of Single Oxygen Molecules with Fluorescence-Labeled Hemocyanins

Sven SdorraWolfgang ErkerThomas Basché

subject

Stereochemistrymedicine.medical_treatmentchemistry.chemical_elementBiochemistryOxygenCatalysisColloid and Surface ChemistrySpecies SpecificityChemical affinitymedicineFluorescence microscopeAnimalsMoleculeFluorescent DyesChemistrySpidersHemocyaninGeneral ChemistryFluorescenceOxygenSpectrometry FluorescenceHemocyaninsBiophysicsOxygen sensorCopperOxygen binding

description

This study introduces a method to detect individual oxygen molecules by fluorescence microscopy of single hemocyanins. These respiratory proteins from a tarantula bind oxygen with high affinity. A spectrometric signature of the oxygenated protein is transferred to an attached fluorescence label, which can be detected at the single-molecule level. This technique opens new perspectives for the development of small and sensitive oxygen sensors as well as for the investigation of cooperative oxygen binding in respiratory proteins.

yearjournalcountryeditionlanguage
2005-10-20Journal of the American Chemical Society
https://doi.org/10.1021/ja053720k