6533b828fe1ef96bd12890bd

RESEARCH PRODUCT

Cytotoxicity, antibacterial and physicochemical properties of a new epoxy resin-based endodontic sealer containing calcium hydroxide

Fernanda HecksherHenrique Dos Santos AntunesRodrigo Ricci VivanEmmanuel João Nogueira Leal SilvaMarco Antonio Hungaro DuarteSabrina De Castro BrasilVictor Talarico Leal Vieira

subject

Radiodensity0206 medical engineering02 engineering and technologyEnterococcus faecalisOperative Dentistry and Endodontics03 medical and health scienceschemistry.chemical_compound0302 clinical medicineMTT assayCytotoxicityGeneral DentistryRoot Canal ObturationCalcium hydroxidebiologyChemistryResearch030206 dentistryEpoxybiology.organism_classification:CIENCIAS MÉDICAS [UNESCO]020601 biomedical engineeringvisual_artOBTURAÇÃO DO CANAL RADICULARUNESCO::CIENCIAS MÉDICASvisual_art.visual_art_mediumAntibacterial activityNuclear chemistry

description

Background This study evaluated the cytotoxicity, antibacterial and physicochemical properties of a new epoxy resin-based endodontic sealer containing calcium-hydroxide Sealer Plus. AH Plus was used as a reference for comparison. Material and Methods Cytotoxicity evaluation was performed according to ISO-10993-5 specifications using MTT assay to check the 3T3 cells viability at 1- to 4-week periods. Antibacterial activity was evaluated using a direct contact test against Enterococcus faecalis. Radiopacity, solubility and flow evaluations were performed according to ISO-6876/2012 specifications. Setting time was assessed following the ANSI/ADA-standard-n.57. The pH level was measured at 3,24,48,72, and 168 hours. Data were statistically analyzed using t-test. The significance level adopted was P0.05). Direct contact results revealed that both freshly prepared sealers has antibacterial effects against Enterococcus faecalis. However, after 7 days both sealers had lost much of their antibacterial effects. Although AH Plus presented higher radiopacity and flow than Sealer Plus (P0.05). AH Plus showed a higher setting time when compared to Sealer Plus (P<0.05). AH Plus and Sealer Plus showed a neutral pH during all tested periods (P<0.05). Conclusions It can be concluded that Sealer Plus showed suitable properties to be used as an endodontic sealer, comparable with those obtained by AH Plus. Key words:Cytotoxicity, root canal filling materials, root canal obturation.

yearjournalcountryeditionlanguage
2019-11-04
10.4317/jced.56534