Studying the Neurovascular Unit: An Improved Blood–Brain Barrier Model

6533b829fe1ef96bd128987d

RESEARCH PRODUCT

Studying the Neurovascular Unit: An Improved Blood–Brain Barrier Model

Heiko J. Luhmann Christoph R.w. Kuhlmann Christoph M. Zehendner

subject

Cell type Blood–brain barrier Cell Line Tight Junctions Brain ischemia Mice In vivo Live cell imaging Parenchyma Electric Impedance medicine Animals Tight junction Caspase 3 Chemistry Brain Endothelial Cells Membrane Proteins medicine.disease Immunohistochemistry Coculture Techniques Endothelial stem cell medicine.anatomical_structure Neurology Blood-Brain Barrier Biophysics Neurology (clinical)Reactive Oxygen Species Cardiology and Cardiovascular Medicine Neuroscience

description

The blood–brain barrier (BBB) closely interacts with the neuronal parenchyma in vivo. To replicate this interdependence in vitro, we established a murine coculture model composed of brain endothelial cell (BEC) monolayers with cortical organotypic slice cultures. The morphology of cell types, expression of tight junctions, formation of reactive oxygen species, caspase-3 activity in BECs, and alterations of electrical resistance under physiologic and pathophysiological conditions were investigated. This new BBB model allows the application of techniques such as laser scanning confocal microscopy, immunohistochemistry, fluorescent live cell imaging, and electrical cell substrate impedance sensing in real time for studying the dynamics of BBB function under defined conditions.

year	journal	country	edition	language
2009-07-29	Journal of Cerebral Blood Flow & Metabolism

https://doi.org/10.1038/jcbfm.2009.103

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