6533b829fe1ef96bd128997a

RESEARCH PRODUCT

Oligonucleotide probes detect splicing variants insituinDrosophilaembryos

Michael AkamRuben ArteroManuel Pérez-alonso

subject

Messenger RNAanimal structuresBase SequenceTranscription GeneticOligonucleotideMolecular Sequence DataAlternative splicingExonic splicing enhancerOligonucleotides AntisenseBiologyMolecular biologyAlternative Splicingchemistry.chemical_compoundchemistryRNA splicingGeneticsAnimalsDigoxigeninDrosophilaspliceOligonucleotide ProbesDigoxigeninIn Situ HybridizationUltrabithorax

description

We describe a method for the in situ detection of specific splicing variants. The method is based on the use of antisense oligonucleotides designed to span splice junctions labelled with digoxigenin by terminal transferase tailing. We find that the spatial patterns of Ubx splicing variants Ia and IIa are similar in early embryos, but differ in late embryos. Variant IVa is only detected in the CNS (ps6) at stages 16 and 17. We also present evidence indicating that the first splicing event is cotranscriptional.

yearjournalcountryeditionlanguage
1992-11-11Nucleic Acids Research
https://doi.org/10.1093/nar/20.21.5687