6533b829fe1ef96bd12899a1

RESEARCH PRODUCT

Low-protein diet prevents tissue lipoprotein lipase activity increase in growing rats

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The time course of changes in tissue lipolytic activities was studied in young rats during theconsumption of a low-protein diet containing 50 g protein/kg (40 g wheat gluten +10 g casein/kg) for 28 d followed by balanced refeeding with 200 g protein/kg (160 g wheat gluten +40 gcasein/kg) for 28 d. Lipoprotein lipase (LPL) activities were compared with the values of acontrol group fed a balanced diet containing 200 g protein/kg for 56 d. At the end of proteinmalnutrition period, the epididymal fat tissue LPL activity represented 36 %, and that of heartand gastrocnemius was 44 %, of those of the control group. These differences wereaccompanied by lower serum- and VLDL-triacylglycerols (TAG), respectively 47·6 % and 31% of the control group values, probably resulting from reduced synthesis of VLDL-apolipo-proteins (29 % of control group values), concomitant with liver lipid accumulation (4·8-fold)and little lipid storage in epididymal fat tissue. At day 2 of refeeding, there was no significantdifference in liver and epididymal fat tissue LPL activities between experimental and controlrats. At the end of the refeeding period, LPL activity of epididymal fat and liver lipolytic activityhad increased and became similar to control group values. The consumption of a low-proteindiet prevented the increase in extrahepatic LPL activities as observed in the control group. Thealterations in LPL activity suggest that a low-protein diet limits lipid storage in adipose tissuedue to reduced serum VLDL-TAG availability.Lipoprotein lipase: Protein malnutrition: Balanced refeeding: Rat