Ex Vivo Treatment with a Polyphenol-Enriched Cocoa Extract Ameliorates Myocardial Infarct and Postischemic Mitochondrial Injury in Normotensive and Hypertensive Rats

6533b829fe1ef96bd1289b71

RESEARCH PRODUCT

Ex Vivo Treatment with a Polyphenol-Enriched Cocoa Extract Ameliorates Myocardial Infarct and Postischemic Mitochondrial Injury in Normotensive and Hypertensive Rats

Susana M. Mosca Juliana Catalina Fantinelli José Luis Ríos Luisa Fernanda González Arbeláez Claudia I. Caldiz Alejandro Ciocci Pardo Guillermo Raúl Schinella

subject

Male 0301 basic medicine Myocardial Infarction Wistar Blood Pressure 030204 cardiovascular system & hematology Pharmacology Mitochondrial Membrane Transport Proteins Infarct size SHR Glycogen Synthase Kinase 3 chemistry.chemical_compound 0302 clinical medicine MITOCHONDRIA Ischemia Superoxides Enos Rats Inbred SHR biology Superoxide MPTP INFARCT SIZE Heart //purl.org/becyt/ford/3.1 [https]Glutathione Mitochondria Medicina Básica Hypertension //purl.org/becyt/ford/3 [https]General Agricultural and Biological Sciences Perfusion Coca Cardiotonic Agents CIENCIAS MÉDICAS Y DE LA SALUD Inmunología Ischemia In Vitro Techniques 03 medical and health sciences POLYPHENOLS WISTAR medicine Animals Humans Rats Wistar SHR; Wistar; infarct size; mitochondria; polyphenols Mitochondrial Permeability Transition Pore Plant Extracts Myocardium Cocoa Extract Polyphenols General Chemistry Glutathione medicine.disease biology.organism_classification Rats 030104 developmental biology chemistry Mitochondrial permeability transition pore Ciencias Médicas

description

Our objective was to determine the effects of a polyphenol-enriched cocoa extract (PCE) on myocardial postischemic alterations in normotensive (Wistar rats, W) and spontaneously hypertensive rats (SHR). Isolated hearts were submitted to 110 min of perfusion or 20 min stabilization, 30 min global ischemia, and 60 min reperfusion (R). Other hearts were treated with PCE at the onset of R. Infarct size, the reduced glutathione (GSH), and the expression of phospho-Akt, P-GSK-3β, and P-eNOS were assessed. In isolated mitochondria, the Ca2+-mediated response of mitochondrial permeability transition pore (mPTP), membrane potential (δψm), and superoxide production were determined. PCE decreased infarct size, partly preserved GSH, increased the P-Akt, P-GSK-3β, and P-eNOS contents, improved mPTP response to Ca2+, decreased the superoxide production, and restored δψm. These data show that PCE decreases the cardiac postischemic damage in W rats and SHR and suggest that Akt/GSK-3β/eNOS dependent pathways are involved.

year	journal	country	edition	language
2016-06-01	Journal of Agricultural and Food Chemistry

https://doi.org/10.1021/acs.jafc.6b01669