6533b829fe1ef96bd128a425

RESEARCH PRODUCT

Impact of histidine spacing on modified polyhistidine tag – Metal ion interactions

Aleksandra HecelJoanna WątłyHenryk KozłowskiMagdalena Rowinska-zyrek

subject

Alanine010405 organic chemistryMetal ions in aqueous solutionSequence (biology)010402 general chemistry01 natural sciences0104 chemical sciencesInorganic ChemistryMetalCrystallographychemistry.chemical_compoundchemistryvisual_artMaterials Chemistryvisual_art.visual_art_mediumOrganic chemistryNarrow rangePhysical and Theoretical ChemistryPolyhistidine-tagHistidine

description

Abstract Histidine rich sequences are chosen both by nature and by molecular biologists due to their high affinity towards metal ions. In this work, we examine the affinity and binding modes of Cu 2+ , Ni 2+ and Zn 2+ towards two histidine tags, the common His 6 -tag (Ac-HHHHHH-NH 2 ) and its modified sequence, which also contains six histidines, but separated with two alanine residues (Ac-HAAHAAHAAHAAHAAHAA-NH 2 ). The spatial separation of histidines has an important impact on its coordination properties. Cu 2+ and Ni 2+ complexes with Ac-HHHHHH-NH 2 are more stable than those with Ac-HAAHAAHAAHAAHAAHAA-NH 2 ; the contrary is observed for Zn 2+ . In a narrow range of pH, Cu 2+ -Ac-HHHHHH-NH 2 and Ni 2+ -Ac-HHHHHH-NH 2 can even compete with the albumin-like binding of the respective metals.

yearjournalcountryeditionlanguage
2018-03-01Inorganica Chimica Acta
10.1016/j.ica.2017.06.053https://doi.org/10.1016/j.ica.2017.06.053