Changes in Tear Protein Pattern after Photorefractive Keratectomy

6533b82afe1ef96bd128c1a6

RESEARCH PRODUCT

Changes in Tear Protein Pattern after Photorefractive Keratectomy

Ildikó Süveges L. Cervenak Franz H. Grus Erika Maka P. Kiszel Amarilla Veres Zoltán Zsolt Nagy Béla Csákány ÁGnes Füst

subject

Adult Immunoglobulin A Pathology medicine.medical_specialty medicine.medical_treatment Eye disease Lacrimal gland Photorefractive Keratectomy Cornea 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cornea Ophthalmology Humans Medicine Postoperative Period Eye Proteins Gel electrophoresis biology business.industry Lactoferrin Lacrimal Apparatus General Medicine Middle Aged medicine.disease eye diseases Photorefractive keratectomy Ophthalmology medicine.anatomical_structure chemistry Tears 030221 ophthalmology & optometry biology.protein Electrophoresis Polyacrylamide Gel Lasers Excimer sense organs Lysozyme business 030217 neurology & neurosurgery

description

PURPOSE. Changes in tear protein composition of patients who underwent photorefractive keratectomy (PRK) were analyzed. METHODS. Tear samples were obtained from 23 eyes of 23 patients immediately before PRK and on the fourth postoperative day with glass capillaries. Tear proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Digital image analysis and evaluation of the densitometric data of the electrophoretic separations were done with BioDoc-Analyze. RESULTS. Analysis of discriminance found a significant difference in the protein patterns (p<0.001). This type of analysis of the electrophoretic densitographs uses all peak information simultaneously. A significant decrease (p<0.005) in three of the main protein peaks - lactoferrin, immunoglobulin A heavy chain, and lysozyme - was also found after PRK. CONCLUSIONS. Excimer laser ablation of the cornea has an acute effect on lacrimal gland protein secretion. Changes in tear composition may lead to feelings of dryness and to a decrease in tear film stability postoperatively.

year	journal	country	edition	language
2003-07-01	European Journal of Ophthalmology

https://doi.org/10.1177/112067210301300603

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