6533b82afe1ef96bd128c47b

RESEARCH PRODUCT

In situ generation of Co(II) by use of a solid-phase reactor in an FIA assembly for the spectrophotometric determination of penicillamine

B. Gómez-taylor CorominasM. Catalá IcardoJulia PferzschnerL. Lahuerta ZamoraJ. Martínez Calatayud

subject

Clinical BiochemistryPharmaceutical Sciencechemistry.chemical_elementAnalytical ChemistryMatrix (chemical analysis)AbsorbanceFIAFlow injection analysisSpectrophotometryDrug DiscoveryQUIMICA ANALITICAmedicineSpectroscopyFlow injection analysisDetection limitChromatographymedicine.diagnostic_testChemistrySpectrum AnalysisPenicillaminePenicillamineReproducibility of ResultsCobaltSolid-phase reactorReagentPharmaceuticalsCobaltmedicine.drug

description

[EN] A flow injection analysis (FIA) manifold for the determination of penicillamine in pharmaceutical preparations is proposed. The manifold includes a solid-phase reactor for the in situ production of the derivatizing reagent, Co(II) ion, which forms a coloured complex with penicillamine in an alkaline medium. The reactor is prepared by natural immobilization of cobalt carbonate on a polymer matrix, which endows it with a high mechanical and microbiological stability. The cobalt released by passage of a 5 x 10(-4) Mol l(-1) sulphuric acid stream at a flow-rate of 2.3 ml min(-1) is merged with a volume of 314 mu l of sample containing penicillamine in ammonium-ammonia buffer at pH 9.5 to measure the absorbance at 360 nm. Beer's law is obeyed over the penicillamine concentration range 5-60 mg l(-1). The limit of detection (LOD) of the method is 1 mg l(-1) and its throughput 70 samples h(-1). (c) 2005 Elsevier B.V. All rights reserved.

yearjournalcountryeditionlanguage
2005-09-01
10.1016/j.jpba.2005.02.044http://hdl.handle.net/10251/54497