A multiplex SNP typing approach for the DNA pyrosequencing technology

6533b82afe1ef96bd128cb9d

RESEARCH PRODUCT

A multiplex SNP typing approach for the DNA pyrosequencing technology

Denise Syndercombe-court C. Nehlich Klaus Bender Peter M. Schneider C. Harrison E. Musgrave-brown

subject

Genetics Pyrosequencing SNP Snapshot (computer storage)Single-nucleotide polymorphism Multiplex Human genome General Medicine Typing Biology SNP genotyping

description

Abstract We have developed a multiplex Pyrosequencing assay which enables the simultaneous analyses of 23 single nucleotide polymorphisms (SNPs) from the human genome selected by the SNPforID Consortium. In our investigations we have studied the multiplex capacity of the PSQ™ 96MA instrument (Biotage AB). To test the reliability of SNP typing by Pyrosequencing the SNPs were analysed in parallel by using the SNaPshot minisequencing technique as reference method.

year	journal	country	edition	language
2006-04-01	International Congress Series

https://doi.org/10.1016/j.ics.2005.12.031

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