6533b82bfe1ef96bd128d771
RESEARCH PRODUCT
Poly-l-Lactic Acid Nanofiber-Polyamidoamine Hydrogel Composites: Preparation, Properties, and Preliminary Evaluation as Scaffolds for Human Pluripotent Stem Cell Culturing.
Elisabetta RanucciChiara GualandiAmedea ManfrediAnna LiguoriMaurilio SampaolesiMaurilio SampaolesiNora BloiseRomolo LauritaPaolo FerrutiNicolò MauroNicolò MauroMaria Letizia FocareteVittorio ColomboLivia VisaiMatteo Gherardisubject
Materials Chemistry2506 Metals and AlloysPluripotent Stem CellsAgmatinePolymers and PlasticsDouble bondpolyamidoaminesPolyestersCell Culture TechniquesNanofibersBioengineering02 engineering and technology010402 general chemistry01 natural sciencesBiomaterialsPolyamidoaminePolyaminesMaterials ChemistryHydrogel compositehuman pluripotent stem cellHumansatmospheric pressure nonequilibrium plasmaInduced pluripotent stem cellatmospheric pressure nonequilibrium plasma; electrospun poly-l-lactic nanofibers; human pluripotent stem cells; poly-l-lactic acid-AGMA1 hydrogel composites; polyamidoamines; biotechnology; bioengineering; biomaterials; polymers and plastics; materials chemistry2506 metals and aloyschemistry.chemical_classificationAddition reactionPolymers and PlasticAqueous solutionTissue ScaffoldsHydrogels021001 nanoscience & nanotechnologyBiomaterial0104 chemical sciencesChemical engineeringchemistryCovalent bondNanofiberelectrospun poly-l-lactic nanofiberpoly-l-lactic acid-AGMA1 hydrogel compositeAmine gas treating0210 nano-technologyBiotechnologydescription
Electrospun poly-l-lactic acid (PLLA) nanofiber mats carrying surface amine groups, previously introduced by nitrogen atmospheric pressure nonequilibrium plasma, are embedded into aqueous solutions of oligomeric acrylamide-end capped AGMA1, a biocompatible polyamidoamine with arg-gly-asp (RGD)-reminiscent repeating units. The resultant mixture is finally cured giving PLLA-AGMA1 hydrogel composites that absorb large amounts of water and, in the swollen state, are translucent, soft, and pliable, yet as strong as the parent PLLA mat. They do not split apart from each other when swollen in water and remain highly flexible and resistant, since the hydrogel portion is covalently grafted onto the PLLA nanofibers via the addition reaction of the surface amine groups to a part of the terminal acrylic double bonds of AGMA1 oligomers. Preliminary tested as scaffolds, the composites prove capable of maintaining short-term undifferentiated cultures of human pluripotent stem cells in feeder-free conditions.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2016-01-01 |