6533b82bfe1ef96bd128dfac
RESEARCH PRODUCT
Determination of phenobarbital in plasma by micellar liquid chromatography
Yolanda Martín-bioscaM.j. Medina-hernándezRosa María Villanueva-camañasSalvador Sagradosubject
PharmacologyDetection limitChromatographyButabarbitalChemistryClinical BiochemistryAnalytical chemistryGeneral MedicinePlasmaBiochemistryAnalytical ChemistryMicellar liquid chromatographyPhase (matter)Drug DiscoverymedicineSample preparationCentrifugationPhenobarbitalMolecular Biologymedicine.drugdescription
A new liquid chromatographic procedure for the determination of phenobarbital in plasma samples is described. The proposed system uses a Spherisorb octadecyl-silane ODS-2 C(18) analytical column, a guard column of similar characteristics, and a 0.03 M CTAB-3% 1-propanol at pH 7 mobile phase. The UV detector was set at 250 nm. Butabarbital was used as internal standard. Sample preparation only required the addition to the plasma samples of a 0.1 M SDS solution at pH 3 and centrifugation before injection into the chromatographic system. The limit of detection was 0.83 microg/mL of phenobarbital in plasma samples. The coefficients of variation were lower than 7. 5%.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2000-04-01 | Biomedical Chromatography |