6533b82bfe1ef96bd128e2d7
RESEARCH PRODUCT
Cytotoxic effect of As(III) in Caco-2 cells and evaluation of its human intestinal permeability.
Ana PolacheDinoraz VélezRosaura FarréLuis GraneroReyes BarberáJosé Moisés LaparraRosa Montorosubject
Intestinal permeabilityArsenic toxicityGlutathione DisulfideChemistryGeneral MedicineGlutathioneToxicologymedicine.diseaseMolecular biologyIntestinal epitheliumGlutathionePermeabilityArsenicMitochondriachemistry.chemical_compoundBiochemistryPermeability (electromagnetism)Caco-2Mediated transportmedicineHumansCaco-2 CellsIntestinal MucosaOxidation-ReductionCarcinogendescription
Inorganic arsenic has been classified as a carcinogen for humans (Group I). However, its transit across the human intestinal epithelium has not been characterized. Using Caco-2 cells, the thiol-redox balance and apparent permeability coefficients (P(app)) for As(III) in the apical to basolateral (AP-BL) and basolateral to apical (BL-AP) direction were evaluated. After As(III) exposure, GSH-induced synthesis was observed, increasing the GSH/GSSG ratio by elevating the As(III) concentration. The AP-BL permeabilities decreased as the As(III) concentrations increased, indicating the existence of a mediated transport mechanism. The (BL-AP)/(AP-BL) permeability ratios were higher than unity, suggesting the existence of a secretion process.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2005-05-17 | Toxicology in vitro : an international journal published in association with BIBRA |