6533b82bfe1ef96bd128e2e0
RESEARCH PRODUCT
Tissue evacuated during joint replacement procedure as a source of mononuclear cells.
Briede IevaMuiznieks IndrikisValdis GoncarsPatetko LieneErglis KristapsErglis AndrejsRasma DortaneSimona KrapseErglis MartinsJakobsons EriksBeatrise Rupainesubject
MalePathologymedicine.medical_specialtyJoint replacementmedicine.medical_treatmentArthroplasty Replacement HipPopulationCD34Antigens CD34OsteoarthritisPunctures030204 cardiovascular system & hematologyPeripheral blood mononuclear cellIliac crestTransplantation AutologousMonocytesOsteoarthritis HipIlium03 medical and health sciences0302 clinical medicinemedicineCentrifugation Density GradientHumansOrthopedics and Sports MedicineBone marrow030212 general & internal medicineExcised tissueeducationArthroplasty Replacement Kneeeducation.field_of_studybusiness.industryCell extractionJoint replacementTissue ProcessingMiddle AgedOsteoarthritis Kneemedicine.diseaseFlow CytometryCell countCell yieldmedicine.anatomical_structureMononuclear cellsCase-Control StudiesTissue and Organ HarvestingSurgeryFemaleBone marrowbusinessOriginal Article • HIP - ARTHROPLASTYdescription
Background Different cell populations from bone marrow were used in various clinical trials for cardiac diseases during last decade. Four clinical studies are ongoing in our institution and enroll patients with cardiac diseases, coronary disease, type 2 diabetes, and osteoarthritis. The density gradient is used to separate bone marrow mononuclear cells. Joint replacement procedures were associated with significant loss of tissue. Usually, excess tissue as bone marrow, peripheral blood and fat are removed to clean operation site. The aim of this study is to prove whether removed tissue during joint replacement procedure can be considered as a significant source of mononuclear cells. Methods Excised tissue obtained during joint replacement procedure was collected by AutoLog system. Bone marrow tissue was collected by iliac crest puncture. Mononuclear cells from both sources were isolated by using Ficoll density gradient centrifugation. Flow cytometry was used to detect mononuclear cell, CD34+ population counts and cell viability. Tissue processing yields between the group of joint replacement and iliac crest puncture group were compared. Results Together, 34 bone marrow tissue processings were performed. On average, samples contained 46.31 ± 9.35 ml of bone marrow solution. Average cell yield in final product was 28.64 ± 9.35 × 106 MNCs and 0.77 ± 1.51 × 106 CD34+ population. In case of tissue removed during joint replacement nine processings were performed. On average samples contained 450 ± 157.69 ml of tissue solution. Average cell yield in final product was 76.67 ± 35.42 × 106 MNCs and 1.33 ± 0.97 × 106 CD34+ population. Conclusions Tissue processing analysis shows that tissue removed during joint replacement procedure can be assumed as a significant source of mononuclear cells. Methods used for bone marrow-derived mononuclear cell extraction can be applied to the excess tissue.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-11-01 | European journal of orthopaedic surgerytraumatology : orthopedie traumatologie |