6533b82cfe1ef96bd128fef5

RESEARCH PRODUCT

Generation of a novel next-generation sequencing-based method for the isolation of new human papillomavirus types

Girts SkendersSankhadeep DuttaTarik GheitAdam GrundhoffAnna R. GiulianoMarcis LejaDaiga SantareMassimo TommasinoNicole FischerAlexis RobitailleRosario Nicola BrancaccioDana E. RollisonCyrille Cuenin

subject

0301 basic medicineGenotypeComputational biologyBiologyOral cavityPolymerase Chain ReactionArticleDNA sequencinglaw.inventionCohort Studies03 medical and health scienceslawVirologyHumansProspective StudiesPapillomaviridaePapillomaviridaePolymerase chain reactionDNA PrimersSkinHuman papillomavirus typesMouthHpv typesPapillomavirus InfectionsHigh-Throughput Nucleotide SequencingSequence Analysis DNAIsolation (microbiology)biology.organism_classificationBiological materials030104 developmental biologyDNA Viral

description

Abstract With the advent of new molecular tools, the discovery of new papillomaviruses (PVs) has accelerated during the past decade, enabling the expansion of knowledge about the viral populations that inhabit the human body. Human PVs (HPVs) are etiologically linked to benign or malignant lesions of the skin and mucosa. The detection of HPV types can vary widely, depending mainly on the methodology and the quality of the biological sample. Next-generation sequencing is one of the most powerful tools, enabling the discovery of novel viruses in a wide range of biological material. Here, we report a novel protocol for the detection of known and unknown HPV types in human skin and oral gargle samples using improved PCR protocols combined with next-generation sequencing. We identified 105 putative new PV types in addition to 296 known types, thus providing important information about the viral distribution in the oral cavity and skin.

yearjournalcountryeditionlanguage
2018-07-01Virology
https://doi.org/10.1016/j.virol.2018.04.017