6533b82cfe1ef96bd1290181
RESEARCH PRODUCT
Resolution improvements in integral microscopy with Fourier plane recording
Bahram JavidiJorge Sola-pikabeaAnabel LlavadorGenaro SaavedraManuel Martínez-corralsubject
PhysicsMicroscopebusiness.industryResolution (electron density)3D reconstruction02 engineering and technologyImage plane021001 nanoscience & nanotechnology01 natural sciencesAtomic and Molecular Physics and Opticslaw.invention010309 opticssymbols.namesakeOpticsFourier transformlawLight sheet fluorescence microscopy0103 physical sciencessymbolsDepth of field0210 nano-technologybusinessImage resolutiondescription
Abstract: Integral microscopes (IMic) have been recently developed in order to capture the spatial and the angular information of 3D microscopic samples with a single exposure. Computational post-processing of this information permits to carry out a 3D reconstruction of the sample. By applying conventional algorithms, both depth and also view reconstructions are possible. However, the main drawback of IMic is that the resolution of the reconstructed images is low and axially heterogeneous. In this paper, we propose a new configuration of the IMic by placing the lens array not at the image plane, but at the pupil (or Fourier) plane of the microscope objective. With this novel system, the spatial resolution is increased by factor 1.4, and the depth of field is substantially enlarged. Our experiments show the feasibility of the proposed method.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2016-09-09 | Optics Express |