0000000000246277
AUTHOR
Jorge Sola-pikabea
THREE-DIMENSIONAL INTEGRAL MICROSCOPY WITH ENHANCED RESOLUTION AND DEPTH OF FIELD
In this contribution we explain two new techniques developed by our group, which permit to increase the two-dimensional spatial resolution of the computed depth images in integral microscopy.
Display of travelling 3D scenes from single integral-imaging capture
Integral imaging (InI) is a 3D auto-stereoscopic technique that captures and displays 3D images. We present a method for easily projecting the information recorded with this technique by transforming the integral image into a plenoptic image, as well as choosing, at will, the field of view (FOV) and the focused plane of the displayed plenoptic image. Furthermore, with this method we can generate a sequence of images that simulates a camera travelling through the scene from a single integral image. The application of this method permits to improve the quality of 3D display images and videos.
Full-parallax 3D display from the hole-filtered depth information
In this paper we introduce an efficient hole-filling algorithm for synthetic generation of microimages that are displayed on an integral imaging monitor. We apply the joint bilateral filter and the median filter to the captured depth map. We introduce in any step of the iterative algorithm with the data from a new Kinect capture. As a result, this algorithm can improve the quality of the depth maps and remove unmeasured depth holes effectively. This refined depth information enables to create a tidy integral image, which can be projected into an integral imaging monitor. In this way the monitor can display 3D images with continuous views, full parallax and abundant 3D reconstructed scene fo…
Breakthroughs in Photonics 2014: Recent Advances in 3-D Integral Imaging Sensing and Display
Integral imaging concept provides a fascinating tool for the capture and display of 3-D images or movies with full parallax, with no need for special glasses and without visual fatigue due to the convergence and accommodation conflict. The limitations and recent advances in integral imaging are discussed.
Resolution improvements in integral microscopy with Fourier plane recording
Abstract: Integral microscopes (IMic) have been recently developed in order to capture the spatial and the angular information of 3D microscopic samples with a single exposure. Computational post-processing of this information permits to carry out a 3D reconstruction of the sample. By applying conventional algorithms, both depth and also view reconstructions are possible. However, the main drawback of IMic is that the resolution of the reconstructed images is low and axially heterogeneous. In this paper, we propose a new configuration of the IMic by placing the lens array not at the image plane, but at the pupil (or Fourier) plane of the microscope objective. With this novel system, the spa…
Integral Imaging Monitors with an Enlarged Viewing Angle
Enlarging the horizontal viewing angle is an important feature of integral imaging monitors. Thus far, the horizontal viewing angle has been enlarged in different ways, such as by changing the size of the elemental images or by tilting the lens array in the capture and reconstruction stages. However, these methods are limited by the microlenses used in the capture stage and by the fact that the images obtained cannot be easily projected into different displays. In this study, we upgrade our previously reported method, called SPOC 2.0. In particular, our new approach, which can be called SPOC 2.1, enlarges the viewing angle by increasing the density of the elemental images in the horizontal …
Integral imaging with Fourier-plane recording
Integral Imaging is well known for its capability of recording both the spatial and the angular information of threedimensional (3D) scenes. Based on such an idea, the plenoptic concept has been developed in the past two decades, and therefore a new camera has been designed with the capacity of capturing the spatial-angular information with a single sensor and after a single shot. However, the classical plenoptic design presents two drawbacks, one is the oblique recording made by external microlenses. Other is loss of information due to diffraction effects. In this contribution report a change in the paradigm and propose the combination of telecentric architecture and Fourier-plane recordin…
Optimal design of incoherent tunable-frequency structured illumination microscope scheme
Structured illumination microscopy (SIM) improves resolution and optical sectioning capability compared to conventional widefield techniques. The main idea of this method is the illumination of the sample with a structured pattern of fixed spatial modulation frequency. Previously, a Fresnel biprism has been implemented in a structured illumination (SI) device providing tunable-frequency sinusoidal patterns. However, the use of this SI system introduces a tradeoff between the visibility and field of view of the illumination fringes. In this contribution, we analyze theoretically this tradeoff and propose the optimal design for the Fresnel biprism-based SIM system.
Fast and robust phase-shift estimation in two-dimensional structured illumination microscopy.
A method of determining unknown phase-shifts between elementary images in two-dimensional Structured Illumination Microscopy (2D-SIM) is presented. The proposed method is based on the comparison of the peak intensity of spectral components. These components correspond to the inherent structured illumination spectral content and the residual compo- nent that appears from wrongly estimated phase-shifts. The estimation of the phase-shifts is carried out by finding the absolute maximum of a function defined as the normalized peak intensity difference in the Fourier domain. This task is performed by an optimization method providing a fast estimation of the phase-shift. The algorithm stability an…
Evaluation of the use of wavefront encoding to reduce depth-induced aberration in structured-illumination microscopy
Three-dimensional imaging is affected by depth-induced spherical aberration (SA) when imaging deep into an optically thick sample. In this work, we evaluate the impact of SA on the performance of incoherent grating-projection structured illumination microscopy (SIM). In particular, we analyze the reduction of the contrast in the structured pattern and compare the reconstructed SIM images for different amounts of SA. In order to mitigate the impact of SA, we implement and evaluate in SIM a wavefront encoded imaging system using a square cubic (SQUBIC) phase mask, an approach shown previously to be successful in conventional microscopy.
FIMic: design for ultimate 3D-integral microscopy of in-vivo biological samples
In this work, Fourier integral microscope (FIMic), an ultimate design of 3D-integral microscopy, is presented. By placing a multiplexing microlens array at the aperture stop of the microscope objective of the host microscope, FIMic shows extended depth of field and enhanced lateral resolution in comparison with regular integral microscopy. As FIMic directly produces a set of orthographic views of the 3D-micrometer-sized sample, it is suitable for real-time imaging. Following regular integral-imaging reconstruction algorithms, a 2.75-fold enhanced depth of field and [Formula: see text]-time better spatial resolution in comparison with conventional integral microscopy is reported. Our claims …
3D Integral Microscopy based in far-field detection
Lately, Integral-Imaging systems have shown very promising capabilities of capturing the 3D structure of micro- scopic and macroscopic scenes. The aim of this work is to provide an optimal design for 3D-integral microscopy with extended depth of field and enhanced lateral resolution. By placing an array of microlenses at the aperture stop of the objective, this setup provides a set of orthographic views of the 3D sample. Adopting well known integral imaging reconstruction algorithms it can be shown that the depth of field as well as spatial resolution are improved with respect to conventional integral microscopy imaging. Our claims are supported on theoretical basis and experimental images …